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oai:figshare.com:article/34041284 2026-10-01T07:24:55Z category_37339 category_37354 category_37366 portal_1012 item_type_1 month_year_10_2026

Supplementary Figure 6 from The H63D Polymorphism in the <i>HFE</i> Gene Promotes Aggressive Disease Phenotypes in Pancreatic Ductal Adenocarcinoma Federica Rubbino (25155083) Luana Greco (25155086) Alessandro Scagliotti (25155089) Alessio Di Cristofaro (25155092) Valentina de Murtas (25155095) Gabriele De Simone (25155098) Stefania Forciniti (25155101) Fabio Grizzi (15125329) Paolo Bianchi (25155104) Gianluca Basso (15137545) Luca Lambroia (25155107) Paolo Kunderfranco (15137566) Paola Cappello (15089919) Francesco Novelli (25155110) Claudio Doglioni (25155113) Piergiuseppe Colombo (25155116) Massimiliano Mazzone (15136493) Massimo Falconi (25155119) Alessandro Zerbi (24694352) Alberto Malesci (25155122) Luigi Laghi (25155125) Cancer Molecular and Cellular Biology Clinical Research and Trials Cell Cycle Control of cell cycle progression Gastrointestinal Cancers Pancreatic cancer Precision Medicine <p>(A) qRT-PCR screening of three independent <i>HFE</i>-targeting siRNAs (siRNA1–3), a pooled siRNA mixture, scramble control, and untreated control in MiaPaCa2 cells (WT for <i>HFE</i> mutation). Relative <i>HFE</i> transcript levels are shown as ΔCt values normalized to 18S.(B) qRT-PCR screening of the same siRNA panel in PANC-1 cells (H63D carriers). (C) Representative Western blot showing HFE and TWIST1 protein expression in MiaPaCa2, A8184, PANC-1, PANC-1 + <i>HFE</i> siRNA, and PANC-1 + scramble. GAPDH was used as loading control. (D) Densitometric quantification of HFE protein levels normalized to GAPDH. (E) Relative mRNA expression of EMT-transcription factor genes (CDH1, CDH2, TWIST1, ZEB1, ZEB2) in wild-type PDAC cell lines (A8184 and MiaPaCa2) following <i>HFE</i> silencing or control treatment. (F) Relative mRNA expression of cell-cycle regulators (<i>CCNC, CCND1, CCNE, CCNA, CCNB</i>) in A8184 and MiaPaCa2 cells after <i>HFE</i> knockdown or control treatment. (G) Densitometric quantification of TWIST1 protein levels normalized to GAPDH. (H) Cell-cycle phase distribution of PANC-1 cells (homozygous H63D) under control conditions or following HFE siRNA treatment. (I) Relative mRNA expression of EMT-related genes (<i>CDH1, SLUG, TWIST, ZEB2, SNAIL</i>) in synchronized PANC-1 cells analyzed in G1 and G2 phases under untreated, scramble, or <i>HFE</i>-silenced conditions. Values are presented as median ±SD. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.</p> 2026-10-01T00:00:00Z Image Figure 10.1158/1078-0432.34041284 https://figshare.com/articles/figure/Supplementary_Figure_6_from_The_H63D_Polymorphism_in_the_i_HFE_i_Gene_Promotes_Aggressive_Disease_Phenotypes_in_Pancreatic_Ductal_Adenocarcinoma/34041284 CC BY