2026-10-06T11:06:24Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34039740 2026-10-01T05:43:24Z category_427 portal_316 item_type_3 month_year_10_2026

Table 1_Viral metagenomic characterization of HAdV-B-related signals in bronchoalveolar lavage fluid from pediatric and adult clinical cohorts.xlsx Chunyan Zhao (141856) Jiaheng Chen (23097283) Ping Wu (46628) Wen Zhang (7555) Songyi Ning (11685460) Public Health and Health Services not elsewhere classified bronchoalveolar lavage fluid HAdV-B human adenovirus metagenomic next-generation sequencing pediatric pneumonia <p>Human adenovirus (HAdV) is an important respiratory virus in children, but sequence-level evidence for HAdV-related signals in lower respiratory tract samples remains limited. In this study, we analyzed metagenomic next-generation sequencing data from 189 bronchoalveolar lavage fluid (BALF) libraries from pediatric and adult clinical cohorts, including 30 pediatric and 159 adult patients, together with laboratory environmental controls. MEGAN read-level classification was used to screen for Adenoviridae-related signals. HAdV-related contigs were further reviewed, and a BALF-derived HAdV-B-related representative contig was selected for read recruitment and coverage analysis. Phylogenetic trees were reconstructed using penton base, hexon, and fiber protein sequences. Adenoviridae-classified signals were more apparent in pediatric BALF libraries than in adult BALF libraries or environmental controls. Several pediatric BALF libraries showed broad read coverage across the HAdV-B-related representative contig, whereas adult libraries and environmental controls mainly showed low-coverage-breadth signals. Phylogenetic analysis placed BALF-derived penton base, hexon, and fiber sequences within HAdV-B-related lineages. Twenty-seven capsid protein sequences from 12 pediatric samples had best BLASTp matches to references annotated as HAdV-B3 (n = 23) or HAdV-B7 (n = 4). These findings provide sequence evidence for HAdV-B-related sequence signals in clinically selected pediatric BALF samples and support further investigation using targeted assays and genome sequencing.</p> 2026-10-01T05:43:24Z Dataset Dataset 10.3389/fpubh.2026.1961482.s003 https://figshare.com/articles/dataset/Table_1_Viral_metagenomic_characterization_of_HAdV-B-related_signals_in_bronchoalveolar_lavage_fluid_from_pediatric_and_adult_clinical_cohorts_xlsx/34039740 CC BY 4.0