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oai:figshare.com:article/34039455 2026-10-01T05:39:29Z category_12 portal_316 item_type_3 month_year_10_2026

Supplementary file 1_Human acellular amniotic membrane loaded with hair follicle-derived cells promotes wound repair and appendage-like structure formation.pdf Yanxin Jiang (8733015) Zhongwen Hou (25152822) Xichao Jian (25152825) Zeyao Tang (3139047) Yan Zhou (6523) Xing Hu (115781) Chengliang Deng (10529766) Shune Xiao (4109980) Cell Biology biological scaffold dermal papilla cells hair follicle stem cells human acellular amniotic membrane wound healing Background<p>Deep skin injuries often heal with scarring and lack skin appendage regeneration, posing a significant clinical challenge. This study aimed to develop an hAAM-based combinatorial strategy for co-delivery of human hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs) to full-thickness skin wounds, and to evaluate its effects on wound repair and appendage-like structure formation.</p>Methods<p>hAAM was prepared and characterized. HFSCs and DPCs were isolated from human hair follicles and identified. The composite construct (hAAM + HFSCs + DPCs) was applied to full-thickness skin wounds in nude mice. Wound healing rates were monitored. Histological and immunohistochemical analyses were performed to assess re-epithelialization, collagen deposition, the presence of CD31-positive vascular structures, cell proliferation, fibrosis-related markers, and morphological features suggestive of appendage-like structures.</p>Results<p>The hAAM scaffold co-loaded with HFSCs and DPCs was associated with accelerated wound closure, tissue repair, and altered expression of fibrosis-associated markers, including TGF-β and α-SMA, together with altered collagen deposition. The treatment was also associated with the appearance of PPAR-γ-positive structures with morphological features resembling sebaceous glands, as well as epithelial bud-like structures morphologically suggestive of early follicular development, in a subset of specimens. Although these morphological observations are preliminary and require further characterization with additional lineage-specific markers, they represent preliminary morphological observations of appendage-like structures.</p>Conclusion<p>The hAAM scaffold co-loaded with HFSCs and DPCs was associated with accelerated wound closure, tissue repair, and altered extracellular matrix remodeling in a murine full-thickness wound model. Structures with morphological features resembling sebaceous glands and epithelial bud-like structures suggestive of early follicular development were observed in some samples; however, their cellular identity, maturity, and functionality remain to be determined. Further lineage-specific characterization and long-term functional studies are required to establish whether this strategy can promote appendage-like structure formation and functional skin restoration.</p> 2026-10-01T05:39:29Z Dataset Dataset 10.3389/fcell.2026.1947836.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Human_acellular_amniotic_membrane_loaded_with_hair_follicle-derived_cells_promotes_wound_repair_and_appendage-like_structure_formation_pdf/34039455 CC BY 4.0