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oai:figshare.com:article/34039311 2026-10-01T05:37:36Z category_8 portal_316 item_type_3 month_year_10_2026

Table 1_Exploration of the mechanisms and active compounds of Scutellaria barbata D. Don against influenza A (H1N1) virus.xlsx Ying Yang (24175) Junjing Shao (25152744) Lin Hou (177757) Shihao Sun (3766798) Guihong Qi (18000811) Microbiology active compounds antiviral H1N1 mechanisms Scutellaria barbata D. Don Introduction<p>Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicinal herb widely used for its heat-clearing and detoxifying properties, has shown broad-spectrum antiviral potential. However, its anti-H1N1 activity and underlying mechanisms remain to be fully elucidated.</p>Methods<p>In this study, the anti-H1N1 activity and underlying mechanisms of S. barbata extract were investigated using H1N1-infected MDCK cells and BALB/c mice. Transcriptomic analysis followed by mRNA and protein-level validation was performed to characterize its molecular effects, while UPLC-Q-Orbitrap-MS was used to identify blood-absorbed constituents. Potential active compounds were subsequently screened by integrating the absorbed constituents with network pharmacology and further validated in vitro and in vivo.</p>Results<p>S. barbata extract markedly inhibited H1N1 replication in MDCK cells (IC₅₀ = 42.87 μg/mL; CC₅₀ > 500 μg/mL) and reduced viral burden and pulmonary inflammation in infected mice. Mechanistically, S. barbata treatment suppressed the Toll-like receptor, NF-κB, and chemokine signaling pathways, suggesting coordinated regulation of antiviral and inflammatory responses. Among the blood-absorbed constituents, baicalein and chrysoeriol were identified as key candidate active compounds and exhibited potent anti-H1N1 activity in vitro and in vivo (IC₅₀ = 13.80 and 11.10 μM, respectively).</p>Discussion<p>Collectively, these findings demonstrate that S. barbata exerts anti-H1N1 effects through a combination of direct inhibition of viral replication and modulation of excessive inflammatory responses, with baicalein and chrysoeriol representing important contributors to its antiviral activity.</p> 2026-10-01T05:37:36Z Dataset Dataset 10.3389/fmicb.2026.1945077.s001 https://figshare.com/articles/dataset/Table_1_Exploration_of_the_mechanisms_and_active_compounds_of_Scutellaria_barbata_D_Don_against_influenza_A_H1N1_virus_xlsx/34039311 CC BY 4.0