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oai:figshare.com:article/34039113 2026-10-01T05:29:12Z category_12 portal_316 item_type_3 month_year_10_2026

Data Sheet 1_Mechanistic study on components of Angelica Pubescens-Taxillus Chinensis in improving compression-induced intervertebral disc degeneration via piezo1/NF-κB signaling axis.pdf Fei Yang (46361) Chaoqun Feng (2382136) Guangye Li (3178725) Junwen Deng (1920052) Yunchuan Gou (25152471) Bingyi Yang (633876) Ying Zhang (40767) Xin Zhou (54275) Chuan Leng (1556569) Maoyin Luo (25152474) Rigao Chen (10350693) Xiaohong Fan (280663) Cell Biology angelica pubescens-taxillus chinensis component-based Chinese medicine NF-κB nucleus pulposus Piezo1 senescence Background<p>Abnormal mechanical load is a critical risk factor for intervertebral disc degeneration (IVDD), and nucleus pulposus (NP) cell senescence is a core pathogenic mechanism in IVDD. Piezo1 is a mechanosensitive ion channel, whereas the NF-κB pathway promotes cellular senescence. The classical formula Duhuo Jisheng decoction has shown potential benefits in IVDD-related conditions, and its core herbal pair Angelica Pubescens-Taxillus Chinensis accounting for its pivotal therapeutic effects.</p>Objective<p>This study aimed to identify the optimal concentration ratio of the Angelica Pubescens-Taxillus Chinensis component-based Chinese medicine (CBCM) for attenuating compression-induced NP cell senescence and elucidate its molecular mechanism.</p>Methods<p>An in vitro NP cell senescence model was established using 1.0 MPa compression. Nine CBCM combinations were defined using an L9 (3³) orthogonal design. A CCK-8 assay was used to screen the optimal and suboptimal combinations. Subsequently, the effect of CBCM on senescence was evaluated using senescence-associated β-galactosidase staining, Western blotting of senescence-related proteins, and qRT-PCR analysis of senescence-associated secretory phenotype factors. Additionally, the impact of CBCM on the Piezo1 and NF-κB pathways was analyzed through Western blotting, qRT-PCR, and immunofluorescence double staining. The underlying mechanism was confirmed by rescue experiments employing a Piezo1 agonist and antagonist, as well as p65 knockdown and overexpression. In vivo, a rat tail disc compression-induced IVDD model was established. The CBCM was administered intraperitoneally. IVDD was evaluated via X-ray, MRI, and histology. Molecular mechanisms underlying senescence, ECM degradation, and the Piezo1/NF-κB axis were analyzed by ELISA and Western blotting. Molecular docking was performed to further validate plausible interactions of CBN, OST, and QR with Piezo1.</p>Results<p>Compression effectively induced NP cell senescence and activated Piezo1 expression and NF-κB signaling. The CBCM concentration ratio that optimally suppressed senescence markers was quercetin at 50 μM, osthole at 6.25 μM, and columbianadin at 12.5 μM. Mechanistically, the optimized CBCM inhibited Piezo1 expression, NF-κB activation, and p65 nuclear translocation. Both p65 knockdown and Piezo1 inhibition mimicked CBCM’s anti-senescent effects, whereas p65 overexpression or Piezo1 agonism reversed these protective effects. These in vitro findings were corroborated in a rat tail compression model of IVDD. Molecular docking offered computational evidence supporting the potential direct binding of CBN, OST, and QR with Piezo1 and proteins involved in the NF-κB signaling pathway.</p>Conclusion<p>These findings suggest that the optimal Angelica Pubescens-Taxillus Chinensis CBCM attenuates the compression-induced senescence-associated phenotype of NP cells in vitro and in a rat tail model by modulating Piezo1-mediated NF-κB signaling.</p> 2026-10-01T05:29:12Z Dataset Dataset 10.3389/fcell.2026.1935597.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Mechanistic_study_on_components_of_Angelica_Pubescens-Taxillus_Chinensis_in_improving_compression-induced_intervertebral_disc_degeneration_via_piezo1_NF-_B_signaling_axis_pdf/34039113 CC BY 4.0