2026-10-11T11:14:29Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34039080 2026-10-01T05:29:03Z category_12 portal_316 item_type_3 month_year_10_2026

Data Sheet 1_Actin disruption is associated with altered YAP/TAZ-responsive transcription and reduced migration during palatal shelf elevation.docx Yang Bai (198601) Jiarong Liu (8703150) Siqi Tang (3998222) Yuxin Zhang (168579) Chang Liu (35901) Fangchun Chen (20146157) Xiaoming Wang (187053) Xiaoxiao Pang (7205189) Cell Biology actin cytoskeleton CCN1 CCN2 cell migration mechanotransduction palatal development YAP/TAZ Introduction<p>Palatal shelf elevation requires coordinated mesenchymal remodeling, but the relationship between actin organization, mechanosensitive transcription, and mesenchymal motility remains incompletely defined. This study examined how actin disruption relates to YAP/TAZ‐responsive transcription and palatal mesenchymal behavior.</p>Methods<p>Primary mouse embryonic palatal mesenchymal cells and E13.5 palate organ cultures were treated with latrunculin A (Lat‐A). Actin organization, YAP localization, wound closure, and palatal shelf orientation were assessed. GA‐017, a LATS1/2 inhibitor that can activate both YAP and TAZ, was used to examine responses in the Lat‐A background. RNA sequencing and reanalysis of public single‐cell multiomic and spatial transcriptomic datasets characterized the associated transcriptional programs.</p>Results<p>Lat‐A disrupted filamentous actin, reduced wound closure, and altered YAP localization. Reduced wound closure persisted in the presence of mitomycin. GA‐017 partially opposed the Lat‐A‐associated changes in wound closure and transcription. RNA sequencing identified 674 genes with opposite-direction changes in the Lat‐A‐versus-control and Lat‐A‐plus‐GA‐017‐versus‐Lat‐A comparisons, including Ccn1, Ccn2, Ankrd1, and Amotl2. In organ culture, Lat‐A was associated with reduced F‐actin, YAP, CCN1, and CCN2 signals and abnormal palatal shelf orientation. Public datasets provided complementary evidence for the developmental expression and spatial overlap of YAP/TEAD‐, CCN‐, actin‐, and migration-related programs.</p>Discussion<p>These findings link actin disruption to altered LATS‐sensitive YAP/TAZ‐responsive transcription and reduced palatal mesenchymal wound closure during palatal elevation. CCN1 and CCN2 are candidate components of this response.</p> 2026-10-01T05:29:03Z Dataset Dataset 10.3389/fcell.2026.1962374.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Actin_disruption_is_associated_with_altered_YAP_TAZ-responsive_transcription_and_reduced_migration_during_palatal_shelf_elevation_docx/34039080 CC BY 4.0