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oai:figshare.com:article/34038507 2026-10-01T04:33:51Z category_363 portal_316 item_type_3 month_year_10_2026

Table 1_Cross-dataset transcriptomics–proteomics analysis identifies Tlr2, Anxa5, and Grin2a as candidate mouse-brain signatures associated with chronic cerebral hypoperfusion.docx Xiang Fan (280830) Meng-Yu Zhong (25152195) Huan Liu (281351) Hao Tao (9729995) Yi Li (1144) Xun Wu (382798) Ying-jie Li (3508574) Bing-Hu Li (281246) Neng-Wei Yu (12052316) Neurology and Neuromuscular Diseases BCAS biomarkers chronic cerebral hypoperfusion proteomics transcriptomics Background<p>Chronic cerebral hypoperfusion (CCH) is a neurological disorder characterized by persistent insufficiency of cerebral blood flow, yet specific biomarkers and mechanistic insights are lacking.</p>Methods<p>This study integrated transcriptomic and proteomic multi-omics analyses, along with behavioral assessments and immunofluorescence validation in a bilateral common carotid artery stenosis (BCAS) mouse model, to identify and validate candidate multi-omics biomarkers of CCH and explore their potential druggability. Leveraging public transcriptomic datasets and in-house proteomic data, differential expression analysis, protein-protein interaction network construction, and five topological algorithms identified Tlr2, Anxa5, and Grin2a as candidate biomarkers.</p>Results<p>BCAS mice exhibited spatial memory impairments in behavioral tests. Immunofluorescence revealed upregulated TLR2 and ANXA5, downregulated GRIN2A, and increased TLR2-IBA1 co-localization indicating microglial activation. Tlr2 and Anxa5 positively correlated with infiltrating immune cells, whereas Grin2a showed a negative correlation. A putative ceRNA network and in silico molecular docking suggested strong binding of pirinixic acid to both TLR2 and ANXA5.</p>Discussion<p>Tlr2, Anxa5, and Grin2a can serve as candidate multi-omics signature molecules of CCH in the mouse brain, with consistent alterations at the transcriptomic, proteomic, and tissue levels.</p> 2026-10-01T04:33:51Z Dataset Dataset 10.3389/fneur.2026.1911864.s001 https://figshare.com/articles/dataset/Table_1_Cross-dataset_transcriptomics_proteomics_analysis_identifies_Tlr2_Anxa5_and_Grin2a_as_candidate_mouse-brain_signatures_associated_with_chronic_cerebral_hypoperfusion_docx/34038507 CC BY 4.0