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oai:figshare.com:article/34037778 2026-10-01T02:06:56Z category_4 category_7 category_12 category_13 category_14 category_19 category_873 category_46 category_734 category_61 category_132 category_133 category_135 portal_63 item_type_6 month_year_10_2026

γ‑Aminobutyric Acid Alleviates Discoloration of Or Mutant Cauliflower via BoMYB24-Mediated Transcriptional Regulation of BoLCYB Wen-zheng Gao (25147554) Xi-zhu Wang (25147557) Yong-hong Ge (25147560) Feng Luo (47640) Biochemistry Medicine Cell Biology Genetics Molecular Biology Pharmacology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Developmental Biology Infectious Diseases Plant Biology Computational Biology transcriptome analysis revealed transcriptional activation effect mediated transcriptional regulation identify key genes action remains unclear candidate transcription factor >) mutant cauliflower bolcyb </ italic mutant cauliflower transcription level cauliflower treated thus delaying positively regulating overexpression increases orange (< markedly upregulated effectively delay commercial value aminobutyric acid abscisic acid 1 motif Orange (<i>Or</i>) mutant cauliflower is prone to discoloration during storage, which affects its commercial value. γ-Aminobutyric acid (GABA) treatment can effectively delay the discoloration of <i>Or</i> mutant cauliflower during storage; however, its mechanism of action remains unclear. Here, we aimed to identify key genes and candidate transcription factors associated with discoloration in <i>Or</i> cauliflower treated with GABA during storage. Transcriptome analysis revealed that GABA regulated <i>BoLCYB</i>, with BoMYB24 identified as the candidate transcription factor in this pathway. BoMYB24 binds the <i>BoLCYB</i> promoter through the MBS-1 motif. Additionally, under GABA treatment, the transcriptional activation effect of BoMYB24 on <i>BoLCYB</i> is further enhanced. <i>BoMYB24</i> overexpression increases <i>BoLCYB</i> expression, leading to a large accumulation of β-carotene in chromoplasts and promoting the accumulation of auxin and abscisic acid. Furthermore, under GABA treatment, the transcription level of BoMYB24 was markedly upregulated, thus delaying the postharvest discoloration of <i>Or</i> mutant cauliflower by positively regulating <i>BoLCYB</i>. 2026-09-30T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c05051.s001 https://figshare.com/articles/journal_contribution/_Aminobutyric_Acid_Alleviates_Discoloration_of_Or_Mutant_Cauliflower_via_BoMYB24-Mediated_Transcriptional_Regulation_of_BoLCYB/34037778 CC BY-NC 4.0