2026-10-12T03:20:47Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34036178 2026-09-30T17:44:52Z category_7 category_12 category_21 category_46 category_64 category_132 category_134 portal_5 item_type_1 month_year_09_2026

Comparison of CXC- Chemokine Expression in Response to IFNγ. Angelina Zimenko (25145435) Ian Hayman (25145438) Youyi Fong (519951) Luke D. Monroe (5165948) Margaret G. Mills (3756298) Alex Greninger (11084570) Christine Johnston (375038) Lawrence Corey (140058) Jia Zhu (135506) Medicine Cell Biology Biotechnology Immunology Cancer Infectious Diseases Virology strongly induced gene protein levels compared limited genital lesions infects genital epithelia increased nuclear localization including robust induction ifn &# 947 enhanced chemokine production immune cell recruitment epithelial antiviral defense antiviral immune cells asymptomatic viral shedding doses much lower cell cytokine signaling epithelial cells efficient recruitment either asymptomatic wide spectrum vaccine strategies understanding variability symptomatic donors results suggest results revealed recurrent self previously reported interferon gamma improving therapies clinical outcomes chemokines involved Primary keratinocytes derived from individuals with asymptomatic or ulcerative HSV-2 disease were mock-treated or treated with increasing concentrations of IFNγ (0.1 to 100 U/mL) for 4 hours (A and B). Log fold change in gene expression of CXCL9, CXCL10, and CXCL11 was calculated relative to untreated group and normalized to β-actin. (A) Individual donor (colored dotted lines) and average donor gene induction (solid black line) of CXCL9, CXCL10, and CXCL11 in response to IFNγ by primary donor keratinocytes. (B) Fold change in CXCL9, CXCL10, and CXCL11 expression. (C and D) Cells were mock-treated or treated with 1.0 or 100 U/mL IFNγ for 12 hours, then CXCL9, CXCL10, and CXCL11 protein was detected in cell culture supernatant using Luminex assay. (C) Bars represent average mean protein per donor cohort; symbols represent means of individual donors. Dotted line for C and D represents a calculated limit of detection. (D) Each line represents an individual donor’s response to 0 U, 1 U and 100 U/mL IFNγ 12 hours post treatment (A-D) Blue bars represent asymptomatic cohort and magenta bars represent ulcerative cohort. *P < 0.05, ** P < 0.01, *** P < 0.001, NS, not significant; linear mixed models were used to analyze fold change in gene expression. N = 5 donors, 3 replicates per donor, per cohort. 2026-09-30T17:55:35Z Image Figure 10.1371/journal.ppat.1014612.g005 https://figshare.com/articles/figure/_p_Comparison_of_CXC-_Chemokine_Expression_in_Response_to_IFN_p_/34036178 CC BY 4.0