2026-10-13T02:13:08Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34036157
2026-09-30T17:44:45Z
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<p>Pretreatment with IFNγ inhibits viral gene expression and replication in keratinocytes from asymptomatic donors and donors with ulcerative disease.</p>
Angelina Zimenko (25145435)
Ian Hayman (25145438)
Youyi Fong (519951)
Luke D. Monroe (5165948)
Margaret G. Mills (3756298)
Alex Greninger (11084570)
Christine Johnston (375038)
Lawrence Corey (140058)
Jia Zhu (135506)
Medicine
Cell Biology
Biotechnology
Immunology
Cancer
Infectious Diseases
Virology
strongly induced gene
protein levels compared
limited genital lesions
infects genital epithelia
increased nuclear localization
including robust induction
ifn &# 947
enhanced chemokine production
immune cell recruitment
epithelial antiviral defense
antiviral immune cells
asymptomatic viral shedding
doses much lower
cell cytokine signaling
epithelial cells
efficient recruitment
either asymptomatic
wide spectrum
vaccine strategies
understanding variability
symptomatic donors
results suggest
results revealed
recurrent self
previously reported
interferon gamma
improving therapies
clinical outcomes
chemokines involved
<p>Keratinocytes were pretreated with specified doses of IFNγ for 24 hours, then infected with either HSV-1 K26 (A), HSV-2 HG52 del47 (B), or HSV-2 186 (C). Gene expression was quantified in (A) and (B) using the Incucyte live cell imagining platform to capture expression of virus-encoded GFP. Total viral genomes were calculated by qPCR from the combined supernatant and cell lysates for HSV-2 186 (C). All data were collected at 24 hours post infection from n = 4 donors per cohort and 3 biological replicates per donor.</p> <p>(TIF)</p>
2026-09-30T17:55:35Z
Image
Figure
10.1371/journal.ppat.1014612.s002
https://figshare.com/articles/figure/_p_Pretreatment_with_IFN_inhibits_viral_gene_expression_and_replication_in_keratinocytes_from_asymptomatic_donors_and_donors_with_ulcerative_disease_p_/34036157
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