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oai:figshare.com:article/34036077 2026-09-30T17:43:20Z category_8 category_12 category_13 category_14 category_24 category_272 category_39 category_734 category_62 category_132 portal_5 item_type_1 month_year_09_2026

<p>Inversion breakpoints of alternatively fixed inversions between <i>D. montana</i> and <i>D. flavomontana</i> pools.</p> Noora Poikela (6613469) Rhonda R. Snook (3260964) Jonna Kulmuni (415890) Michael G. Ritchie (7876421) Microbiology Cell Biology Genetics Molecular Biology Evolutionary Biology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Marine Biology Infectious Diseases stressful thermal conditions potentially mitigating climate natural populations using inverted autosomal regions higher male fertility global temperatures rise div >< p colinear autosomal regions certain genomic regions avoid local extinctions tightly link adaptive three overlapping inversions strong species barrier containing barrier loci adaptive genetic material also mapped long may resist introgression introgression occurred also improved heat tolerance improve heat tolerance heat waves become heat stress compared >, exposing heat standing genetic barriers maintaining control lines barrier loci genetic barriers three generations thereby maintaining adaptive alleles control lines adaptive introgression strong selection x chromosome term barriers study demonstrates sequence experiment reduced recombination rapid source primarily located montana </ less pronounced gene flow flavomontana </ findings highlight <p>Illumina paired-end re-sequencing data of both species were mapped against <i>D. flavomontana</i> reference genome, and the data is illustrated with Integrative Genomics Viewer (IGV) [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004028#pbio.3004028.ref082" target="_blank">82</a>]. Due to inversion differences between the species, <i>D. montana</i> reads are split, the insert size of its paired reads deviates from the expected (red reads), and paired reads are in reversed orientation (blue and turquoise reads). Note that not all X inversion breakpoints exhibit reversed orientation due to the complex overlap of these inversions (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004028#pbio.3004028.s001" target="_blank">S1 Table</a>). Since chromosome 4 of the <i>D. flavomontana</i> reference genome was scaffolded, the presence of the inversion on chromosome 4 was verified by mapping both species to the <i>Drosophila lummei</i> reference genome (breakpoints 1,568,294 and 15,689,984 in the <i>D. lummei</i> genome equal to 23,745,872 and 7,855,585 in the <i>D. flavomontana</i> genome, respectively; <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004028#pbio.3004028.s001" target="_blank">S1 Table</a>), as described in Poikela and colleagues [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004028#pbio.3004028.ref050" target="_blank">50</a>]. The data underlying this figure can be found at <a href="https://doi.org/10.5281/zenodo.22277897" target="_blank">https://doi.org/10.5281/zenodo.22277897</a>.</p> <p>(TIF)</p> 2026-09-30T18:02:59Z Image Figure 10.1371/journal.pbio.3004028.s009 https://figshare.com/articles/figure/_p_Inversion_breakpoints_of_alternatively_fixed_inversions_between_i_D_montana_i_and_i_D_flavomontana_i_pools_p_/34036077 CC BY 4.0