2026-10-14T04:18:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34035992 2026-09-30T17:42:49Z category_7 category_12 category_15 category_16 category_873 category_106 category_734 portal_5 item_type_1 month_year_09_2026

Experimental data gathered during prolonged osmotic challenge and recovery. Duncan J. MacGregor (25145402) Medicine Cell Biology Neuroscience Physiology Chemical Sciences not elsewhere classified Science Policy Biological Sciences not elsewhere classified regulate water loss dynamically heterogeneous patterns complete neural system neuronal cell bodies prolonged osmotic challenge peptide hormone vasopressin hypothalamic vasopressin neurons extracellular fluid osmolarity distributed neuroendocrine system stores gradually decline distant hormone stores detailed neuronal model despite upregulated synthesis turn trigger secretion regulate plasma volume maintain hormone supply div >< p 2 +</ sup modelling vasopressin synthesis synthesis dynamics based activity dependent upregulation plasma volume dependent upregulation sustained challenge storage dynamics recovery based neuroendocrine cells model suggests elevated osmolarity neuronal activity large stores coordinate synthesis timescale memory slow recovery several days salt loading posterior pituitary pituitary terminals physiological input mrna content mrna acts intracellular ca homeostatic systems generate complex form part experimentally tested demand balance coupling spiking axonal terminals The data here (available in supporting material <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1014832#pcbi.1014832.s002" target="_blank">S1 Data</a>) is gathered from multiple published sources where rats have been measured during a prolonged osmotic challenge consisting of several days of dehydration (circles) or salt loading (squares), and the following recovery period, with normal water access restored. During the challenge osmolarity and plasma [Na+] (top panels) rise mostly linearly, with some data showing a reduced rate of rise and plateau towards day 4 and 5. Radioimmunoassay measured plasma vasopressin concentration (mid left) mostly shows a matching linear rise, but data is mostly limited to three days, and varies in magnitude between dehydration (higher) and salt loading (lower) protocols. Vasopressin cell body mRNA content (mid right) shows a mostly linear rise after one day that eventually plateaus. The data is variable, but the most consistent experiments, with more time points, suggest a five to eight fold rise in content. The core target data for the model is the measurements of pituitary store content (bottom). During the challenge there is a mostly linear fall in pituitary content, which slows after day 3, falling to around 15–30% of normal content. During recovery, where osmolarity rapidly (a few hours) returns to normal, the stores are slowly replenished over about two weeks. The faster recovery shown here (blue squares) is in rats made hypo-osmotic after the prolonged hyper-osmotic challenge. Detail on the sources (different colours) is given in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1014832#pcbi.1014832.s001" target="_blank">S1 Fig</a>. 2026-09-30T17:55:49Z Image Figure 10.1371/journal.pcbi.1014832.g001 https://figshare.com/articles/figure/_p_Experimental_data_gathered_during_prolonged_osmotic_challenge_and_recovery_p_/34035992 CC BY 4.0