2026-10-14T03:24:46Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34035582 2026-09-30T17:35:09Z category_4 category_12 category_13 category_14 category_734 portal_5 item_type_1 month_year_09_2026

<p>Galactose-induced CDC6 overexpression of <i>ORC4-</i> and <i>ORC1-</i> plasmid-shuffle strains.</p> Luis E. Martínez-Rodríguez (25145346) Stephen P. Bell (25145349) Biochemistry Cell Biology Genetics Molecular Biology Biological Sciences not elsewhere classified origin recognition complex saccharomyces cerevisiae </ helicase loading activity div >< p atpase interfaces within six orc subunits orc atp hydrolysis defective orc allele atp hydrolysis orc subunits defective orc vitro </ toa2 </ r267a </ sixth allele reducing transcription orc4r ). lethal phenotype genetic screen essential role cdc6 complex binds origins although functions <p>Ten-fold serial dilutions of <i>ORC4</i>, <i>orc4-R267A</i>, <i>ORC1</i>, <i>orc1-K485T</i>, and <i>orc1-R616A</i> plasmid-shuffle strains with or without induction of the <i>GAL-CDC6</i> allele. A control strain with a <i>CDC6</i> deletion covered by plasmid with wild-type Cdc6 is also shown (rows 3 and 4). All strains were grown overnight in galactose, plated, and incubated at 30°C for 3 days.</p> 2026-09-30T17:34:54Z Image Figure 10.1371/journal.pone.0349465.g005 https://figshare.com/articles/figure/_p_Galactose-induced_CDC6_overexpression_of_i_ORC4-_i_and_i_ORC1-_i_plasmid-shuffle_strains_p_/34035582 CC BY 4.0