2026-10-12T06:24:05Z
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2026-09-30T17:34:59Z
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<p>Additional biochemical characterization of the ORC4R protein.</p>
Luis E. Martínez-Rodríguez (25145346)
Stephen P. Bell (25145349)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Biological Sciences not elsewhere classified
origin recognition complex
saccharomyces cerevisiae </
helicase loading activity
div >< p
atpase interfaces within
six orc subunits
orc atp hydrolysis
defective orc allele
atp hydrolysis
orc subunits
defective orc
vitro </
toa2 </
r267a </
sixth allele
reducing transcription
orc4r ).
lethal phenotype
genetic screen
essential role
cdc6 complex
binds origins
although functions
<p>A) Comparison of DNA binding of WT ORC, ORC4R with and without the Orc4-<i>sofa</i> mutation, and ORC1K in electrophoretic-mobility shift assay (EMSA). Binding in the presence of ATP or ATPγS is shown. B) Sequence specificity of ORC and ORC4R DNA binding. EMSA was used to measure wild-type ORC and ORC4R DNA binding to WT <i>ARS1</i> or <i>ARS1</i> lacking the strongest ORC binding site (A-). C) Titration of WT ORC, ORC4R, and ORC1K proteins in a helicase-loading assay (0.3 mM ATP). D) Salt titration and sequence-specific loading of the WT and ORC4R protein (R267A). Sequence specificity is tested using a WT <i>ARS1</i> and an <i>ARS1</i> lacking the two strongest ORC binding sites (A-/B2-).</p> <p>(TIF)</p>
2026-09-30T17:34:54Z
Image
Figure
10.1371/journal.pone.0349465.s005
https://figshare.com/articles/figure/_p_Additional_biochemical_characterization_of_the_ORC4R_protein_p_/34035555
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