2026-10-13T21:21:33Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34035396 2026-09-30T17:33:43Z category_7 category_873 category_45 category_734 category_811 category_64 category_105 category_132 category_134 portal_5 item_type_6 month_year_09_2026

<p>Characteristics of serological tests.</p> Nédio Mabunda (679203) Odete Matola (25145319) Júlia Sambo (8748228) Imelda Chelene (4836756) Abras Munguambe (25145322) Nuro Abílio (14724950) Adolfo Vubil (353401) Osvaldo Loquiha (300896) Ilesh Jani (563942) Medicine Chemical Sciences not elsewhere classified Sociology Biological Sciences not elsewhere classified Mathematical Sciences not elsewhere classified Cancer Statistics Infectious Diseases Virology linked immunosorbent assays improve transfusion safety human immunodeficiency virus high transmission risk cobas mpx test 2 western blot 1 %; 70 1 %; 68 determine hiv 1 000 blood donors undetected positive results 7 per 1 4 %, respectively routine hiv testing 5 %; 90 0 %; 66 6 %; 88 gold hiv ). mozambique </ p molecular testing gold hiv controlling hiv blood transfusions blood banks study point study aimed serological tests rapid tests mozambique ), developing countries critical evaluation analytical performance <div><p>Many settings in sub-Saharan Africa use rapid antibody assays to detect human immunodeficiency virus (HIV) infection among blood donor candidates. This study aimed to evaluate the analytical performance of HIV serological tests used to screen blood donor candidates in Mozambique. Determine HIV 1/2 + Uni-Gold HIV (the routine HIV testing at blood banks in Mozambique), and two newer rapid tests (Determine HIV Early Detect and Meriscreen HIV1–2 Western Blot) were compared with an Enzyme-Linked Immunosorbent Assays (Merilisa HIV Gen 4 test + Uni-Gold HIV). Additionally, all serological tests were compared with the molecular cobas MPX test. The sensitivity and specificity of Determine HIV 1/2 + Uni-Gold HIV; Determine HIV Early Detect, and Meriscreen HIV1–2 Western Blot compared to the Merilisa HIV Gen 4 test + Uni-Gold were 85.5% and 99.6%; 88.7% and 99.5%; 90.0% and 99.6% respectively. The sensitivity and specificity of Determine HIV 1/2 + Uni-Gold HIV; Determine HIV Early Detect, Meriscreen HIV1–2 Western Blot, and Merilisa HIV Gen 4 test + Uni-Gold compared to the cobas MPX test were 64.0% and 99.1%; 68.6% and 99.1%; 70.8% and 99.0%; 66.0% and 99.4%, respectively. The probability of undetected positive results in the rapid tests and Merilisa HIV Gen 4 test ranged from 5.6 to 6.7 per 1,000 blood donors. The results of this study point to the need for the introduction of molecular testing for HIV in blood banks in developing countries to improve transfusion safety and contribute to controlling HIV as a public health problem.</p></div> 2026-09-30T17:33:40Z Text Journal contribution 10.1371/journal.pgph.0007398.s002 https://figshare.com/articles/journal_contribution/_p_Characteristics_of_serological_tests_p_/34035396 CC BY 4.0