2026-10-14T10:19:40Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34035007
2026-09-30T17:30:09Z
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month_year_09_2026
<p>LC–MS analysis of OL extract.</p>
Fadwa M. Arafa (25145191)
Mohamed Hagar (7347941)
Ahmed Zakaria (6164612)
Eman Sheta (12250849)
Nehal N. Hezema (25145194)
Biochemistry
Microbiology
Cell Biology
Molecular Biology
Pharmacology
Biotechnology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Ecology
Immunology
Computational Biology
severely deformed ones
scanning electron microscopy
restored villus architecture
new therapeutic avenue
lc – ms
grave risk posed
goblet cell count
div >< p
current research focused
statistically significant rise
serum mda levels
regarding oocyst count
intestinal villi revealed
combating intestinal cryptosporidiosis
similar ultrastructural alterations
bacillus clausii </
th </ sup
intestinal cryptosporidiosis
clausii </
oocyst reduction
mean levels
corresponding rise
vivo </
clausii -</
vulnerable populations
treated group
synergistic potential
proficient use
mass spectrometry
lymphocytic population
liquid chromatography
incensole isomers
immunosuppressed mice
histopathological findings
highest percentage
extensive pits
either alone
day post
complete distortion
combined therapy
chemical composition
best results
>, ranging
6 %),
<div><p>Given the grave risk posed by intestinal cryptosporidiosis to vulnerable populations, the current research focused on a new therapeutic avenue. Olibanum (OL) extract and probiotic <i>Bacillus clausii</i> (<i>B. clausii</i>) and their combination were evaluated against <i>Cryptosporidium in vivo</i> in immunosuppressed mice via parasitological, ultrastructural, biochemical, and histopathological analysis. Liquid chromatography-mass spectrometry (LC–MS) analysis of OL revealed the predominance of incensole isomers in its chemical composition. Regarding oocyst count in stool, the highest percentage of oocyst reduction was achieved after combined therapy (98.4%) on the 19<sup>th</sup> day post-infection, followed by <i>B. clausii-</i>treated group (87.6%), then the OL-treated group (87%). Similar ultrastructural alterations were noticed by scanning electron microscopy (SEM) following treatment by both OL and <i>B. clausii</i>, ranging from shrunken oocysts to severely deformed ones with extensive pits or protrusions. Nonetheless, their combination resulted in complete distortion of the oocysts, leading to their rupture. Biochemically, both OL and <i>B. clausii</i> treatment, either alone or in combination, provoked a decrease in the serum MDA levels, together with a corresponding rise in the mean levels of GSH. Concerning the histopathological findings, the best results were achieved following the combined OL and <i>B. clausii</i> treatment, which has restored villus architecture and remarkably ameliorated detrimental intestinal inflammation and goblet cell count. Finally, immunohistochemical analysis of the lymphocytic population of the intestinal villi revealed a statistically significant rise in the CD4+ /CD8+ ratio after treatment with either OL, <i>B. clausii</i> or their combination, which signals their proficient use for this therapeutic endeavour.</p></div>
2026-09-30T17:29:57Z
Dataset
Dataset
10.1371/journal.pntd.0014734.t001
https://figshare.com/articles/dataset/_p_LC_MS_analysis_of_OL_extract_p_/34035007
CC BY 4.0