2026-10-11T17:57:49Z
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oai:figshare.com:article/34034911
2026-09-30T17:28:55Z
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<p>Low-dose/high-stress exposure trends towards reduced hippocampal neurogenesis and increased astrogliosis in the CA1 and CA3 regions.</p>
Rashelle Lashley (7302038)
Joshua Brian Foster (20175776)
Xueqin Wang (350340)
Albert John Muhleman (20175779)
Samuel Weaver (1344039)
Edison Lin (25145140)
Liching Lai (25145143)
Chien-liang Glenn Lin (16856919)
Neuroscience
Biological Sciences not elsewhere classified
Science Policy
Mental Health
related chemical agents
investigational compound designed
including chronic pain
improve translational rigor
robust behavioral phenotypes
like behavioral phenotypes
standardize animal models
harlan involving exposure
experimental conditions evaluated
gulf war illness
male sd rats
experimental conditions
behavioral assessments
gulf war
exposure paradigms
animal sources
rats using
rats may
subsequently modified
stress levels
rat model
psychological stress
original objective
neuropsychiatric symptoms
mood problems
mild stress
initially attempted
continued efforts
cognitive impairment
better interpret
<p>Following behavioral assessments (the above low-dose/high-stress exposure study), a subset of rats was euthanized to examine markers for astrocytes (GFAP<sup>+</sup>), parvalbumin-positive interneurons (PV<sup>+</sup>), and newborn neurons (DCX<sup>+</sup>) in hippocampal regions using immunohistochemical staining. For each staining, 3–4 animals and at least 3 sections per animal were analyzed. Data were analyzed by two-way ANOVA. <b>(A-C) the GFAP immunoreactivity quantification</b>. DG, n = 27–33; CA1, n = 31–34; CA3, n = 31–36. <b>(D) the number of PV</b><sup><b>+</b></sup> <b>neurons</b>. n = 22–26. <b>(E) the number of DCX</b><sup><b>+</b></sup> <b>newborn neurons</b>. n = 29–39. Two-way ANOVA revealed a significant main effect of the GW exposure for GFAP - CA1, CA3, and DCX – DG; however, Bonferroni post hoc tests showed no significant differences among the four groups.</p>
2026-09-30T17:28:43Z
Image
Figure
10.1371/journal.pone.0337157.g004
https://figshare.com/articles/figure/_p_Low-dose_high-stress_exposure_trends_towards_reduced_hippocampal_neurogenesis_and_increased_astrogliosis_in_the_CA1_and_CA3_regions_p_/34034911
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