2026-10-10T15:53:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34034680 2026-09-30T17:26:53Z category_4 category_12 category_13 category_14 category_15 category_873 category_734 category_64 portal_5 item_type_1 month_year_09_2026

<p>Effects of AZP2006 on TH(+) primary mesencephalic neurons injured by MPP⁺ (4 µM) for 48 h (A-D) or with Rotenone (10 nM) for 24 h (E-H).</p> Noelle Callizot (5660677) Georgia Culley (15148575) Grace Flower (25145065) Laura Rouvière (16400955) Véronique De Conto (25145068) Alexandre Wojcinski (6340172) Catherine Botto (25145071) Cecilia Estrella (1618609) Alexandre Henriques (359791) Philippe Verwaerde (1618624) Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Cancer promising therapeutic approach lewy bodies ). several lysosomal enzymes restoring lysosomal homeostasis lysosomal availability depend like models (< parkinson &# 8217 maintaining lysosomal function div >< p neuroprotective effects involve several parkinson ’ lysosomal function neuroprotective effects lysosomal system lysosomal dysfunction parkinson ’ neuroprotective action disease models vivo </ vitro </ ultimately supporting prone proteins pathohistological hallmarks mitochondrial injury genetic connections endoplasmic reticulum dopaminergic neurons direct link clinical development <p>ICC analysis of cultures shows the total number of TH(+) neurons (A,E), the length of their neurite network (B,F), the area of cytoplasmic α-syn per TH(+) neuron (C,G) and lysosome burden as area of LAMP2(+) vesicles per TH(+) neuron (D and H), expressed as % of control (CTR). Raw values for MPP⁺ & Rotenone CTR (i.e., 100%); sum TH(+) neurons per well, i.e., per biological replicate = 18 ± 0.5 & 26 ± 1; neurite network = 889 ± 57 & 929 ± 24 µm; α-syn area/TH(+) neuron = 29 ± 2.1 & 28 ± 1 µm²; ratio LAMP2/TH(+) = 10 ± 0.3 & 10 ± 0.5, respectively. Each bar represents the mean ± SEM, one-way ANOVA followed by Fisher’s test. ^####p < 0.0001 from CTR and *p < 0.05, **p < 0.01; ***p < 0.001 or ****p < 0.0001 from MPP⁺ or Rotenone treatment, (n = 4-6 / group).</p> 2026-09-30T17:26:42Z Image Figure 10.1371/journal.pone.0359269.g002 https://figshare.com/articles/figure/_p_Effects_of_AZP2006_on_TH_primary_mesencephalic_neurons_injured_by_MPP_sup_sup_4_M_for_48_h_A-D_or_with_Rotenone_10_nM_for_24_h_E-H_p_/34034680 CC BY 4.0