2026-10-12T21:07:55Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34034152 2026-09-30T17:08:43Z category_12 category_13 category_14 category_21 category_873 category_39 category_46 category_61 category_133 portal_63 item_type_6 month_year_09_2026

Vicia faba TEOSINTE BRANCHED1/CYCLOIDEA/PCF Transcription Factors VfTCP19, VfTCP21 and VfTCP23 Contribute to Biotic and Abiotic Stress-Associated Responses Junliang Yin (9184419) Hongguang Zhu (397574) Shi Chen (120917) Xiaohui Yin (547490) Yi Yuan (180239) Yiting Li (2730601) Yongxing Zhu (548368) Cell Biology Genetics Molecular Biology Biotechnology Chemical Sciences not elsewhere classified Ecology Immunology Developmental Biology Plant Biology strong purifying selection strengthening water retention responsive expression patterns regulating stomatal aperture potential candidate genes improving water status adverse environmental conditions >- elements related maintaining ros homeostasis experimental analyses confirmed scavenging ros qpcr confirmed vftcp23 contribute transient overexpression transcriptome profiling sustainable production severely constrained salt tolerance resistance breeding promoters contained pathogen resistance nuclear localization hydrophilic proteins findings revealed enhanced drought cis </ 23 </ Faba bean provides food and feed, also improves field soil. However, its sustainable production is severely constrained by adverse environmental conditions. Identification desired genes could provide resources for resistance breeding, thus improve field performance. Here, 24 VfTCP transcription factors were identified and characterized as nucleus-localized, unstable, hydrophilic proteins. VfTCPs were under strong purifying selection, and their promoters contained <i>cis</i>-elements related to growth, development, and stress responses. Transcriptome profiling and RT-qPCR confirmed their stress-responsive expression patterns. Experimental analyses confirmed the nuclear localization and GAL4-based transcriptional activation activity of <i>VfTCP19/21/23</i>. Transient overexpression of <i>VfTCP19/21/23</i> contributed to pathogen resistance by maintaining ROS homeostasis and attenuating BAX-, NIP-, and INF1-induced. Meanwhile, they enhanced drought and salt tolerance by regulating stomatal aperture, strengthening water retention, improving water status, and scavenging ROS, thereby alleviating leaf wilting. These findings revealed <i>VfTCP19/21/23</i> as potential candidate genes for future stress-resistance breeding. 2026-09-30T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c06084.s001 https://figshare.com/articles/journal_contribution/Vicia_faba_TEOSINTE_BRANCHED1_CYCLOIDEA_PCF_Transcription_Factors_VfTCP19_VfTCP21_and_VfTCP23_Contribute_to_Biotic_and_Abiotic_Stress-Associated_Responses/34034152 CC BY-NC 4.0