2026-10-13T01:41:40Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34032671
2026-09-30T13:39:32Z
category_1
category_146
category_7
category_21
category_873
category_734
portal_63
item_type_6
month_year_09_2026
Effect of Initial
Dissolution Behavior on Cellular
Response in Uncoated and Calcium Phosphate-Coated Magnesium Alloy
Aoife McFerran (25144223)
Adam Griebel (25144226)
Jeremy Schaffer (25144229)
Ellamay McIlhatton (25144232)
John O’Connor (146719)
Joanna Ward (6298553)
Jonathan G. Acheson (25144235)
Biophysics
Space Science
Medicine
Biotechnology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
time points (<
raised ph levels
like mechanical properties
including rr assay
hour deposition times
initial dissolution behavior
sputtered calcium phosphate
2 +</ sup
calcium phosphate
initial corrosion
rapid dissolution
dissolution environment
vivo </
sem study
rf magnetron
resorbable implant
protein adhesion
promising alternative
physiological conditions
p </
ion release
immersion remains
findings demonstrate
effectively delay
early stages
dependent across
dapi staining
critical challenge
corrosion medium
complete degradation
cellular responses
cellular response
alloys offer
5 μm
24 h
001 ),
Biodegradable magnesium (Mg) alloys offer a promising
alternative
to permanent orthopedic implants due to their bone-like mechanical
properties, biocompatibility, and complete degradation <i>in
vivo</i>. However, the rapid dissolution that occurs during the
early stages of immersion remains a critical challenge in their feasibility
as a resorbable implant. In this work, RF magnetron-sputtered calcium
phosphate (CaP) coatings of ∼0.5 μm and ∼1 μm
thickness corresponding to 15- and 30-hour deposition times, respectively,
were applied to a WE22 alloy. The effect on initial dissolution behavior,
ion release, and cellular responses was evaluated when immersed for
1, 7, and 24 h. Uncoated WE22 exhibited rapid dissolution with substantial
Mg<sup>2+</sup> release (∼18 mg/L at 24 h) and raised pH levels
from ∼7 to ∼8.8. While ∼0.5 μm CaP coatings
reduced these effects, they showed greater mass gain and protein adhesion,
suggesting potential coating inhomogeneity or nano-defects trapping
corrosion constituents. The ∼1 μm coating provided notable
corrosion resistance and maintained a dissolution environment most
comparable to physiological conditions. U-2 OS cell viability assessments,
including RR assay, DAPI staining, and SEM study, determined that
cytotoxicity was concentration-dependent across all time points (<i>p</i> < 0.001), with 100% corrosion medium and uncoated WE22
most detrimental to viability. These findings demonstrate the potential
for CaP coatings to effectively delay or control the initial corrosion
of WE22 while providing enhanced biocompatibility compared to the
uncoated substrate.
2026-09-30T00:00:00Z
Text
Journal contribution
10.1021/acsomega.6c04124.s001
https://figshare.com/articles/journal_contribution/Effect_of_Initial_Dissolution_Behavior_on_Cellular_Response_in_Uncoated_and_Calcium_Phosphate-Coated_Magnesium_Alloy/34032671
CC BY-NC 4.0