2026-10-12T08:34:50Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34032555 2026-09-30T13:12:51Z category_303 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Exploratory microbiota-metabolite alterations associated with cognitive impairment after repeated low-dose sevoflurane exposure in mice.docx Hui Tang (226667) Qingqing Xiong (1829848) Gang Qian (1428967) Clinical Microbiology cognitive impairment gut microbiota metabolomics metagenomics sevoflurane Background<p>Repeated low-dose sevoflurane exposure may affect cognition, but the relationship between such exposure, gut microbial composition, and circulating metabolites remains unclear. This study examined an experimental proof-of-concept exposure model and treated microbiota-metabolite findings as exploratory associations.</p>Methods<p>Mice were exposed to 100 ppm sevoflurane for 2 h/day over 7 consecutive days, followed by Morris water maze testing without a long-term recovery follow-up. Gut microbiota and serum metabolites were profiled by shotgun metagenomic sequencing and LC-MS metabolomics, respectively, and machine-learning analyses were used only for exploratory feature ranking.</p>Results<p>Sevoflurane-exposed mice exhibited poorer Morris water maze performance (Day 6 escape latency: 28.6 ± 3.4 s vs. 12.5 ± 1.8 s in controls, p < 0.01), although interpretation is limited by unequal behavioral sample sizes. Serum metabolomics suggested nominal and exploratory alterations in bile acid- and sphingolipid-related pathways. Shotgun metagenomics (n = 3/group) identified preliminary relative-abundance shifts, including a lower Bacteroidota/Bacillota ratio and lower relative abundance of Akkermansia.</p>Conclusions<p>In this proof-of-concept model, repeated low-dose sevoflurane exposure was associated with impaired spatial learning and memory and with exploratory serum metabolic and gut microbial alterations. The findings do not establish persistent long-term deficits, routine occupational risk, or causal microbiota-metabolite mediation. Larger cage-aware studies, an ambient-air comparison group, targeted validation, and interventional experiments are required.</p> 2026-09-30T13:12:51Z Dataset Dataset 10.3389/fcimb.2026.1869293.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Exploratory_microbiota-metabolite_alterations_associated_with_cognitive_impairment_after_repeated_low-dose_sevoflurane_exposure_in_mice_docx/34032555 CC BY 4.0