2026-10-11T13:17:50Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34032098 2026-09-30T12:04:43Z category_1 category_4 category_915 category_12 category_19 category_21 category_873 category_133 category_134 portal_63 item_type_3 month_year_09_2026

A Novel Boranil-Based Colorimetric and Fluorescence Quenching Probe for Selective Detection and Bioimaging of the Fe³⁺ ion Darakhshan Parveen (20854478) Saumya Singh (1567216) Avadhesh Pratap Singh (8197956) Alok Kumar Verma (25143743) Abhishek Kumar (156479) Chandni Singh (11009360) Suryabhan Singh (2158834) Ashish Kumar Singh (206583) Divya Pratap Singh (8197962) Mukesh Kumar (132604) Ajay Kumar (192967) Biophysics Biochemistry Physical Sciences not elsewhere classified Cell Biology Pharmacology Biotechnology Chemical Sciences not elsewhere classified Plant Biology Virology uv – vis toxic metal ions solution color changes resolution mass spectrometry efficient cellular uptake competing metal ions 5 × 10 35 × 10 readily synthesizable boranil fluorescence responses toward b nmr spectroscopy fluorescence quenching probe association constant (< 3 +</ sup 15 </ sup 13 </ sup 11 </ sup 1 </ sup 2 </ sub association constant k </ pronounced quenching ir spectroscopy fluorescence titrations fluorescence signals wide range spectroscopically characterized ray diffraction plot analysis pale yellow novel boranil mixed etoh job ’ including na crystal x biologically relevant binding stoichiometry based colorimetric based chemosensor >< sub 3σ method A novel, readily synthesizable boranil-based chemosensor, HSR, was structurally and spectroscopically characterized by single-crystal X-ray diffraction, IR spectroscopy, ¹H, ¹³C, and ¹¹B NMR spectroscopy, and high-resolution mass spectrometry (HRMS). HSR exhibited highly selective colorimetric and fluorescence responses toward the Fe³⁺ ion over a wide range of competing metal ions, including Na⁺, K⁺, Ba²⁺, Ca²⁺, Mg²⁺, Al³⁺, Zn²⁺, Ni²⁺, Cu²⁺, Co³⁺, Pb²⁺, Cd²⁺, Hg²⁺, and Fe²⁺. Upon Fe³⁺ addition, the solution color changes from pale yellow to red-brown, accompanied by pronounced quenching of fluorescence signals. Concentration-dependent Fe³⁺ ion titrations revealed significant modulation of the photoluminescence and UV–vis absorption profiles. The binding stoichiometry and association constant between HSR and the Fe³⁺ ion were determined by UV–vis and fluorescence titrations, Job’s plot analysis, and HRMS, confirming strong complex formation. Notably, no interference from biologically relevant or toxic metal ions was observed. The limit of detection (LOD), calculated using the 3σ method, was 7.5 × 10^–6 M. The association constant (<i>K</i>_a), obtained from a Benesi–Hildebrand analysis, was 2.35 × 10¹⁵ M^–1, indicating exceptionally strong Fe³⁺ ion binding. Furthermore, HSR displayed good solubility in mixed EtOH/H₂O media and efficient cellular uptake with no cytotoxicity, highlighting its suitability for the detection of the Fe³⁺ ion and bioimaging applications. 2026-09-30T00:00:00Z Dataset Dataset 10.1021/acsomega.6c01069.s001 https://figshare.com/articles/dataset/A_Novel_Boranil-Based_Colorimetric_and_Fluorescence_Quenching_Probe_for_Selective_Detection_and_Bioimaging_of_the_Fe_sup_3_sup_ion/34032098 CC BY-NC 4.0