2026-10-11T18:08:52Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34031329 2026-09-30T10:40:03Z category_13 category_21 category_272 category_873 category_39 category_734 category_69 category_132 portal_87 item_type_3 month_year_09_2026

Development of an integrated multi-omic targeted workflow for Gammarus fossarum: a proof-of-concept application to study endocrine disruption in environmental species Rémy De Boni (25143148) Delphine Arquier (18390399) Melanie Nguyen (14525757) Sophie Ayciriex (3624860) Davide Degli-Esposti (4570378) Olivier Geffard (556813) Arnaud Chaumot (81659) Yohann Clement (25143151) Arnaud Salvador (1712308) Genetics Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry Infectious Diseases Multi-omics extraction Gammarus fossarum Scout-MRM highly targeted mass spectrometry endocrine disruptors developments in analytical chemistry The focus of chemical risk assessment is shifting towards mechanistic system biology in order to better capture subtle effects of environmental contaminants such as endocrine disruptors. However, mechanistic studies in ecologically relevant sentinel species often require organ-level investigation, which is hindered by extreme limitations in biomass and high inter-individual variability. In this context, we developed a targeted multi-omic workflow (proteomics, metabolomics, and lipidomics) optimised for low-mass sentinel species such as Gammarus fossarum. A unified ‘metabolomics-first’ co-extraction protocol enabled the simultaneous recovery of proteins, metabolites, and lipids from identical caeca pools. To overcome data processing bottlenecks of high-resolution mass spectrometry (HRMS) and ensure high sensitivity, a fully targeted mass spectrometry strategy was adopted across all omics layers. Specifically, the Scout-MRM approach was implemented via the Scout-MRM Builder tool for metabolomics, providing an efficient alternative to untargeted HRMS. Meanwhile, proteomics and lipidomics utilised targeted MRM. As a proof of concept, the pipeline was applied to caeca exposed to two crustacean endocrine disruptors : fenoxycarb and tributyltin. Integrated analysis revealed consistent molecular shifts across energy, membrane, and detoxification pathways. These findings, serving as mechanistic hypotheses, demonstrate the potential of this single-sample workflow to provide a synchronised view of organismal physiological state. This work establishes a methodological framework for advancing systems ecotoxicology in non-model species. 2026-09-30T10:40:03Z Dataset Dataset 10.6084/m9.figshare.34031329.v1 https://figshare.com/articles/dataset/Development_of_an_integrated_multi-omic_targeted_workflow_for_i_Gammarus_fossarum_i_a_proof-of-concept_application_to_study_endocrine_disruption_in_environmental_species/34031329 CC BY 4.0