2026-10-11T13:29:19Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34030464 2026-09-30T07:12:39Z category_4 category_8 category_16 category_19 category_272 category_873 category_39 category_734 portal_63 item_type_3 month_year_09_2026

Common Nitrification Inhibitors Exhibit Distinct Mechanisms on the Ammonia-Oxidizing Archaeon Nitrososphaera viennensis Dimitrios Dalkidis (12017142) Andrea Malits (8900387) Melina Kerou (6987863) Hadis Sajedi (25142304) Leila Afjehi-Sadat (2262928) Christa Schleper (27900) Dimitrios G Karpouzas (25142307) Evangelia S Papadopoulou (25142310) Logan H. Hodgskiss (14632220) Biochemistry Microbiology Physiology Pharmacology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified triggered broader shifts soil nitrogen cycling chelating free copper e ., ammonia aoa nitrososphaera viennensis ammonia monooxygenase complex synthetic nitrification inhibitors nitrification inhibitors 3 nitrification inhibitors two inhibitors urea acquisition relief experiments proteomic signals physiological responses physiological assays oxidizing archaea nitrifiers compete limiting step investigated using functional genomics energy metabolism elicits activation dominant drivers diverged markedly dimethylpyrazole phosphate clear transcriptomic central role based fertilizers aob ). agricultural soils additional ammonia Microbial ammonia oxidation, the first and rate-limiting step of nitrification, plays a central role in soil nitrogen cycling. It is most relevant in agricultural soils as nitrifiers compete with crops for ammonia-based fertilizers. Therefore, synthetic nitrification inhibitors are widely used alongside fertilizers to reduce the activities of dominant drivers of this process, i.e., ammonia-oxidizing archaea (AOA) and bacteria (AOB). However, the physiological responses of ammonia oxidizers remain poorly resolved. Here, the response of the AOA Nitrososphaera viennensis to the nitrification inhibitors 3,4-dimethylpyrazole phosphate (DMPP) and allylthiourea (ATU) was investigated using a combination of functional genomics, physiological assays, and relief experiments. The results overturn earlier assumptions that DMPP and ATU act by chelating free copper. Both compounds affected ammonia oxidation and triggered broader shifts in energy metabolism and stress-response pathways, which diverged markedly between the two inhibitors. We propose a competitive inhibition of the ammonia monooxygenase complex with DMPP as it can be alleviated by additional ammonia and elicits activation of urea acquisition, while ATU acted as a noncompetitive inhibitor generally inducing quiescence. Both modes of inhibition were associated with clear transcriptomic and proteomic signals that will be advantageous for the identification of mechanisms of other nitrification inhibitors in the future. 2026-09-30T00:00:00Z Dataset Dataset 10.1021/acs.est.6c08773.s004 https://figshare.com/articles/dataset/Common_Nitrification_Inhibitors_Exhibit_Distinct_Mechanisms_on_the_Ammonia-Oxidizing_Archaeon_Nitrososphaera_viennensis/34030464 CC BY-NC 4.0