2026-10-14T05:36:46Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34030352 2026-09-30T07:00:10Z category_8 category_13 category_14 category_272 category_39 category_734 category_69 category_133 portal_87 item_type_3 month_year_09_2026

Large-scale megabarcoding using Oxford Nanopore Technology enables fine-scale mapping of <i>Terebellides</i> (Annelida, Trichobranchidae) in Sweden with the description of two new species Maël Grosse (23156879) Daria Gaeva (5428991) Paul Amblard Rambert (25142216) Anna Zhadan (23882895) Erica Leder (3250179) Arne Nygren (730912) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry Plant Biology Taxonomy mapping benthos monitoring megabarcoding barcoding biodiversity distribution White Sea Arctic <p>Species of the genus <i>Terebellides</i> (Annelida, Trichobranchidae) are common and abundant in marine benthic communities. They are also morphologically homogeneous, and molecular studies have revealed the presence of many undescribed species in the North-East Atlantic. This can hinder accurate environmental monitoring. In the Skagerrak and Kattegat, ten species have been previously detected based on molecular analyses. However, the number of species occurring in Swedish waters, their distributions and relative abundance are unclear.</p> <p>To resolve these questions, a total of 521 specimens of <i>Terebellides</i> were gathered for molecular analyses. To analyse such a large dataset, two methods were used and compared. The first one used individual Sanger sequencing to obtain a 313 bp fragment of the COI marker. The second method combined tagged primers and multiplexed sequencing with Oxford Nanopore Technology (ONT). A total of 191 sequences were obtained with Sanger sequencing and 413 sequences with ONT, demonstrating the advantage of the latter method for this type of dataset. Six species were found, including <i>Terebellides europaea, T. lavesquei, T. shetlandica</i> and <i>T. williamsae</i> and new specimens of two species that had previously been detected but remained undescribed. They are here described as <i>Terebellides agreniusi</i> sp. nov. and <i>Terebellides nevmyanovi</i> sp. nov.</p> <p>LSID for the publication: <a target="_blank" href="http://urn:lsid:zoobank.org:pub:9D13BD18-9D25-4327-8139-F46273449BD9">urn:lsid:zoobank.org:pub:9D13BD18-9D25-4327-8139-F46273449BD9</a></p> <p>LSID for <i>T. agreniusi:</i> <a target="_blank" href="http://urn:lsid:zoobank.org:act:012F26B8-D297-4F43-9F45-33230BE1B158">urn:lsid:zoobank.org:act:012F26B8-D297-4F43-9F45-33230BE1B158</a></p> <p>LSID for <i>T. nevmyanovi:</i> <a target="_blank" href="http://urn:lsid:zoobank.org:act:261E60E9-265F-457A-BE43-ADFF995A9C3A">urn:lsid:zoobank.org:act:261E60E9-265F-457A-BE43-ADFF995A9C3A</a></p> <p></p><p>Oxford Nanopore Technology is an efficient and cost-effective method for large-scale barcoding and mapping of benthic invertebrates.</p><p>Two new species of <i>Terebellides</i> are described from Russia and Sweden.</p><p>A synoptic table for species of <i>Terebellides</i> present in the Skagerrak and Kattegat is given.</p><p></p> <p>Oxford Nanopore Technology is an efficient and cost-effective method for large-scale barcoding and mapping of benthic invertebrates.</p> <p>Two new species of <i>Terebellides</i> are described from Russia and Sweden.</p> <p>A synoptic table for species of <i>Terebellides</i> present in the Skagerrak and Kattegat is given.</p> 2026-09-30T07:00:10Z Dataset Dataset 10.6084/m9.figshare.34030352.v1 https://figshare.com/articles/dataset/Large-scale_megabarcoding_using_Oxford_Nanopore_Technology_enables_fine-scale_mapping_of_i_Terebellides_i_Annelida_Trichobranchidae_in_Sweden_with_the_description_of_two_new_species/34030352 CC BY 4.0