2026-10-13T20:03:13Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34030293 2026-09-30T06:22:21Z category_1 category_4 category_12 category_13 category_14 category_16 category_873 category_46 category_64 category_69 category_132 portal_63 item_type_6 month_year_09_2026

Fucoidan Alleviates Intestinal Inflammation through Microbiota-Associated Citraconic Acid Accumulation to Modulate Ferroptosis-Related Pathways Yifan Wei (7439075) Xiaomeng Ren (371196) Shuang Song (457799) Chunqing Ai (640882) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Immunology Cancer Inorganic Chemistry Infectious Diseases related oxidative stress improved barrier function candidate metabolite associated associated metabolic changes associated antioxidant responses antibiotic depletion suggested gut microbial profile 2 +</ sup related pathways fucoidan mediated intestinal protection associated ca accumulation mediated protection intestinal protection study investigated shown potential results highlight protective effects potential mediator partially dependent modulate ferroptosis lipid peroxidation inflammatory activity induced colitis identify ca gut microbiota gpx4 expression enhanced nrf2 beneficial taxa attenuated ferroptosis Fucoidan has shown potential for intestinal protection; however, its underlying mechanisms remain unclear. This study investigated the protective effects of fucoidan against DSS-induced colitis and explored microbiota-associated metabolic changes that were linked to its protective effects. Fucoidan alleviated colonic injury, improved epithelial barrier integrity, and reshaped the gut microbial profile, including reduced Pseudomonadota levels and the enrichment of beneficial taxa. Targeted metabolomics identified citraconic acid (CA) as a candidate metabolite associated with fucoidan-mediated protection. Antibiotic depletion suggested that fucoidan-associated CA accumulation was partially dependent on the gut microbiota. CA reduced colitis severity, attenuated ferroptosis-related oxidative stress, and improved barrier function, accompanied by enhanced Nrf2-associated antioxidant responses and the restoration of SLC7A11 and GPX4 expression. In vitro, CA reduced mitochondrial Fe²⁺ accumulation and lipid peroxidation while enhancing tight junction protein expression. These results highlight a microbiota-associated metabolic mechanism underlying fucoidan-mediated intestinal protection and identify CA as a potential mediator of its anti-inflammatory activity. 2026-09-30T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c08698.s001 https://figshare.com/articles/journal_contribution/Fucoidan_Alleviates_Intestinal_Inflammation_through_Microbiota-Associated_Citraconic_Acid_Accumulation_to_Modulate_Ferroptosis-Related_Pathways/34030293 CC BY-NC 4.0