2026-10-12T06:38:31Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34029337 2026-09-30T04:04:01Z category_1 category_4 category_7 category_12 category_13 category_14 category_16 category_734 category_931 category_69 category_132 category_135 portal_63 item_type_6 month_year_09_2026

Quercetin Alleviates TMT-Induced PANoptosis in Chicken Skeletal Muscle through Modulation of the ROS/mtDNA Axis Chengguo Liu (2745184) Ping Zhang (86495) Yue Zhai (3141960) Fangfang Wu (526041) Jiahui Duan (9284688) Tong Xu (762586) Lixia Zhao (174165) Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Physiology Biological Sciences not elsewhere classified Information Systems not elsewhere classified Inorganic Chemistry Infectious Diseases Computational Biology respiratory chain dysfunction recognized systemic toxicity reactive oxygen species network analysis indicated restored mitochondrial homeostasis molecular dynamics simulations mitochondrial dynamic imbalance skeletal muscle effects chicken skeletal muscle associated muscle damage vitro validation showed environmental organotin contaminant mtdna axis trimethyltin quercetin alleviates tmt que alleviates tmt vitro validation muscle injury induced skeletal mtdna axis mitochondrial regulators mitochondrial dna associated proteins sensing pathways remain unclear related signaling protective mechanism oxidative stress molecular docking mediated protection feed additive dietary intervention cytosolic dna Trimethyltin (TMT) is an environmental organotin contaminant with recognized systemic toxicity, but its skeletal muscle effects and the protective mechanism of quercetin (Que) remain unclear. This study integrated network toxicology, molecular docking, molecular dynamics simulations, and in vivo/in vitro validation to investigate Que-mediated protection against TMT-induced skeletal-muscle injury. Network analysis indicated the involvement of oxidative stress and cytosolic DNA-sensing pathways, while docking and dynamic simulations suggested stable interactions between Que and mitochondrial regulators and PANoptosis-associated proteins. In vivo and in vitro validation showed that TMT induced skeletal muscle injury, reactive oxygen species (ROS) accumulation, mitochondrial dynamic imbalance, respiratory chain dysfunction, mitochondrial DNA (mtDNA) leakage, and PANoptosis activation. Que markedly suppressed ROS generation, restored mitochondrial homeostasis, reduced cytosolic mtDNA leakage, and inhibited PANoptosis-related signaling. Collectively, Que alleviates TMT-induced skeletal muscle toxicity by regulating the ROS/mtDNA axis, supporting its potential as a dietary intervention or feed additive against organotin-associated muscle damage. 2026-09-30T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c06054.s001 https://figshare.com/articles/journal_contribution/Quercetin_Alleviates_TMT-Induced_PANoptosis_in_Chicken_Skeletal_Muscle_through_Modulation_of_the_ROS_mtDNA_Axis/34029337 CC BY-NC 4.0