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oai:figshare.com:article/34028597 2026-09-30T02:06:51Z category_1 category_4 category_12 category_14 category_16 category_272 category_39 category_734 portal_63 item_type_6 month_year_09_2026

Multimodal Hyperspectral Imaging Unveils Pigment Rearrangement in the Diatom Cyclotella meneghiniana under Changing Physicochemical Conditions Lena Golubewa (9568366) Yaraslau Padrez (14766515) Patricija Zemaityte (24359690) Paulius Rindzevicius (23245143) Andrej Dementjev (20868011) Kipras Mažeika (25139804) Augustas Vaitkevičius (25139807) Claudia Büchel (1906564) Andrius Gelzinis (7865462) Biophysics Biochemistry Cell Biology Molecular Biology Physiology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified stokes raman scattering photon excited fluorescence peripheral girdle lamella nonresonance cars ratios confocal fluorescence microscopy combines coherent anti spatial pigment distribution cyclotella meneghiniana </ cars ), two absorbing fx accompanied subcellular metabolic responses multimodal framework provides 640 nm emission multimodal framework metabolic responses spatial organization fx ’ c </ aggregation ), possibly fucoxanthin physiological conditions photosynthetic machinery mostly red machine learning lower packing induced rearrangements free tool findings show decreased resonance chlorophyll protein Revealing the spatial organization and stress-induced rearrangements of the photosynthetic machinery in thylakoids of diatoms is crucial for understanding their metabolic responses and/or adaptation. Using a multimodal framework that combines coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence, and confocal fluorescence microscopy with machine learning, we investigated the spatial pigment distribution in thylakoids of <i>Cyclotella meneghiniana</i>. Under physiological conditions, we observed dense central packing of fucoxanthins (Fx’s). In contrast, the peripheral girdle lamella had a lower packing of mostly red-absorbing Fx accompanied by enhanced 710–750 nm chlorophyll (Chl) emission. Glycerol treatment induced thylakoid disintegration, which was reflected in decreased resonance-to-nonresonance CARS ratios (Fx disordering), emergence of a 690–700 nm fluorescence component, and 680 nm band narrowing (possibly fucoxanthin-chlorophyll protein (FCP) aggregation), as well as 640 nm emission (Chl <i>c</i> release). Our findings show that this multimodal framework provides a powerful, label-free tool for mapping the photosynthetic machinery and subcellular metabolic responses. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acs.jpclett.6c02711.s001 https://figshare.com/articles/journal_contribution/Multimodal_Hyperspectral_Imaging_Unveils_Pigment_Rearrangement_in_the_Diatom_Cyclotella_meneghiniana_under_Changing_Physicochemical_Conditions/34028597 CC BY-NC 4.0