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oai:figshare.com:article/34027412 2026-09-29T19:12:13Z category_4 category_7 category_8 category_39 category_133 category_135 portal_63 item_type_6 month_year_09_2026

Unraveling the Functional Diversity of Succinate Dehydrogenase Subunits in Fusarium verticillioides Guiping Zhang (420333) Shuodan Hu (12417211) Qiaowan Chen (17721415) Zhankai Che (25138676) Zhongshou Wu (425577) Yun Chen (279569) Zhonghua Ma (194585) Biochemistry Medicine Microbiology Ecology Plant Biology Computational Biology tricarboxylic acid cycle succinate dehydrogenase subunits maize ear rot exerted distinct effects electron transport chain concurrently reduced sensitivity adaptive fungicide responses anchoring subunits fvsdhc1 sdhi fungicide pydiflumetofen individual sdh subunits fvsdhc1 </ sdhi response sdhi fungicides transcriptional divergence toxin production fvsdhd </ fvsdhc2 </ fvsdhb </ fvsdha </ fvsdh complex fusarium verticillioides fungal development functional diversity duplicated membrane dramatic upregulation coordinated structural causal agent Succinate dehydrogenase (Sdh) is a conserved mitochondrial complex linking the tricarboxylic acid cycle and electron transport chain and is the target of SDHI fungicides. However, the roles of individual Sdh subunits in fungal development and SDHI responses remain unclear. Here, we systematically characterized Sdh subunits in Fusarium verticillioides, the causal agent of maize ear rot. Deletion of <i>FvSdhA</i>, <i>FvSdhB</i>, or <i>FvSdhD</i> severely impaired fungal growth, conidiation, toxin production, pathogenicity, and concurrently reduced sensitivity to the SDHI fungicide pydiflumetofen. In contrast, the duplicated membrane-anchoring subunits FvSdhC1 and FvSdhC2 exhibited partial functional redundancy as deletion of <i>FvSdhC1</i> led to a dramatic upregulation of <i>FvSdhC2</i> transcript, and both could potentially support Sdh complex formation, while they exerted distinct effects on virulence and SDHI response. Together, these findings suggest functional specialization within the FvSdh complex and indicate that FvSdhC paralogs may contribute to adaptive fungicide responses through coordinated structural and transcriptional divergence. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c07102.s001 https://figshare.com/articles/journal_contribution/Unraveling_the_Functional_Diversity_of_Succinate_Dehydrogenase_Subunits_in_Fusarium_verticillioides/34027412 CC BY-NC 4.0