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oai:figshare.com:article/34027139 2026-09-29T17:41:16Z category_915 category_7 category_8 category_13 category_873 category_46 category_734 category_132 category_135 portal_5 item_type_1 month_year_09_2026

<p><i>P. falciparum</i> parasite development in RBCs with different HBB genotypes.</p> Yasmina Drissi-El Boukili (25138150) Vera Kühne (7252466) H. Magloire Natama (25138153) Pieter Moris (5712428) Elisabet Tintó-Font (18572506) Antonia Windkouni Bere (25138156) Odin Goovaerts (138054) Pieter Guetens (1804669) Ana Moreno-Murillo (25138159) Yarno Valgaerts (25138162) T. Edwig Traoré (25138165) D. Florence Ouedraogo (25138168) Halidou Tinto (257156) Ann Van de Velde (22056553) Alfred Cortés (33324) Guy Caljon (119879) Anna Rosanas-Urgell (112252) Physical Sciences not elsewhere classified Medicine Microbiology Genetics Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Infectious Diseases Computational Biology malaria transmission relies influence parasite growth infected rbc antigens sc remains unclear ex vivo </ measure sc rates hbb mutant genotypes different hbb genotypes host hemoglobin beta sc assay (< plasmodium falciparum </ div >< p higher sc rates sc rates falciparum </ sc ). >) sc vitro </ tom </ iv </ ev </ type hbb sexual conversion results demonstrate reporter line process initiated nf54 -< igm responses humoral immunity human natural hbaa ). genetic factor flow cytometry disease outcome burkina faso asexual parasites <p>(A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA <i>n</i> = 1, HbAC <i>n</i> = 1 and HbAS <i>n</i> = 2, and HbSS <i>n</i> = 2 and HbSC <i>n</i> = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages (<i>i.e.,</i> schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA (<i>n</i> = 5), HbAC (<i>n</i> = 5),HbAS (<i>n</i> = 4), and HbSC (<i>n</i> = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS (<i>n</i> = 12), HbSC (<i>n</i> = 1) and <i>n</i> = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.</p> 2026-09-29T17:40:42Z Image Figure 10.1371/journal.ppat.1014542.g003 https://figshare.com/articles/figure/_p_i_P_falciparum_i_parasite_development_in_RBCs_with_different_HBB_genotypes_p_/34027139 CC BY 4.0