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oai:figshare.com:article/34027136 2026-09-29T17:41:15Z category_915 category_7 category_8 category_13 category_873 category_46 category_734 category_132 category_135 portal_5 item_type_1 month_year_09_2026

<p>Effect of HbAA, HbAC, HbAS, HbSS and HbSC on SC by <i>iv</i>SCAs.</p> Yasmina Drissi-El Boukili (25138150) Vera Kühne (7252466) H. Magloire Natama (25138153) Pieter Moris (5712428) Elisabet Tintó-Font (18572506) Antonia Windkouni Bere (25138156) Odin Goovaerts (138054) Pieter Guetens (1804669) Ana Moreno-Murillo (25138159) Yarno Valgaerts (25138162) T. Edwig Traoré (25138165) D. Florence Ouedraogo (25138168) Halidou Tinto (257156) Ann Van de Velde (22056553) Alfred Cortés (33324) Guy Caljon (119879) Anna Rosanas-Urgell (112252) Physical Sciences not elsewhere classified Medicine Microbiology Genetics Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Infectious Diseases Computational Biology malaria transmission relies influence parasite growth infected rbc antigens sc remains unclear ex vivo </ measure sc rates hbb mutant genotypes different hbb genotypes host hemoglobin beta sc assay (< plasmodium falciparum </ div >< p higher sc rates sc rates falciparum </ sc ). >) sc vitro </ tom </ iv </ ev </ type hbb sexual conversion results demonstrate reporter line process initiated nf54 -< igm responses humoral immunity human natural hbaa ). genetic factor flow cytometry disease outcome burkina faso asexual parasites <p><b>(A)</b> Experimental design of <i>in vitro</i> SC assays (<i>iv</i>SCAs) using the NF54<i>-gexp02-Tom P. falciparum</i> line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in <b>(B)</b> +Choline cultures and (C) in -Choline cultures. <b>(D)</b> Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. <b>(E)</b> Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant <i>p</i>-values are shown as: <i>p</i> < 0.05*, <i>p</i> < 0.01**, <i>p</i> < 0.001***; non-significant <i>p</i>-values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, <b>D.</b> (2026) <a href="https://BioRender.com/ew8vbur" target="_blank">https://BioRender.com/ew8vbur</a>.</p> 2026-09-29T17:40:42Z Image Figure 10.1371/journal.ppat.1014542.g002 https://figshare.com/articles/figure/_p_Effect_of_HbAA_HbAC_HbAS_HbSS_and_HbSC_on_SC_by_i_iv_i_SCAs_p_/34027136 CC BY 4.0