2026-10-11T14:36:48Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34027067
2026-09-29T17:40:45Z
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<p>Whole genome sequencing analysis of parasite DNA.</p>
Yasmina Drissi-El Boukili (25138150)
Vera Kühne (7252466)
H. Magloire Natama (25138153)
Pieter Moris (5712428)
Elisabet Tintó-Font (18572506)
Antonia Windkouni Bere (25138156)
Odin Goovaerts (138054)
Pieter Guetens (1804669)
Ana Moreno-Murillo (25138159)
Yarno Valgaerts (25138162)
T. Edwig Traoré (25138165)
D. Florence Ouedraogo (25138168)
Halidou Tinto (257156)
Ann Van de Velde (22056553)
Alfred Cortés (33324)
Guy Caljon (119879)
Anna Rosanas-Urgell (112252)
Physical Sciences not elsewhere classified
Medicine
Microbiology
Genetics
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Infectious Diseases
Computational Biology
malaria transmission relies
influence parasite growth
infected rbc antigens
sc remains unclear
ex vivo </
measure sc rates
hbb mutant genotypes
different hbb genotypes
host hemoglobin beta
sc assay (<
plasmodium falciparum </
div >< p
higher sc rates
sc rates
falciparum </
sc ).
>) sc
vitro </
tom </
iv </
ev </
type hbb
sexual conversion
results demonstrate
reporter line
process initiated
nf54 -<
igm responses
humoral immunity
human natural
hbaa ).
genetic factor
flow cytometry
disease outcome
burkina faso
asexual parasites
<div><p>Malaria transmission relies on the differentiation of asexual parasites into gametocytes, a process initiated by sexual conversion (SC). Mutations in the host hemoglobin beta (HBB) gene are known to influence parasite growth and disease outcome, yet their impact on SC remains unclear. We investigated the effect of HBB mutant genotypes on <i>Plasmodium falciparum</i> (<i>P. falciparum</i>) SC and humoral immunity in individuals from Nanoro, Burkina Faso. To measure SC rates in natural human malaria infections, we developed a new <i>ex vivo</i> SC assay (<i>ev</i>SCA). We found that in human natural <i>P. falciparum</i> infections, SC rates were higher in individuals with HbAS or HbAC than in individuals with wild-type HBB (HbAA). Consistently, using an <i>in vitro</i> SC assay (<i>iv</i>SCA) based on the NF54-<i>gexp02-Tom</i> reporter line we found that cultures grown in HbAS red blood cells (RBCs) had higher SC rates than those grown in HbAA RBCs. Furthermore, IgG and IgM responses against trophozoite- and stage I gametocyte-infected RBC antigens, quantified by flow cytometry, did not differ between plasma from individuals with different HBB genotypes. These results demonstrate that exposure to RBCs with HBB mutations enhances SC, highlighting a host-genetic factor that may influence malaria transmission potential.</p></div>
2026-09-29T17:40:42Z
Text
Journal contribution
10.1371/journal.ppat.1014542.s002
https://figshare.com/articles/journal_contribution/_p_Whole_genome_sequencing_analysis_of_parasite_DNA_p_/34027067
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