2026-10-14T22:24:17Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34026937
2026-09-29T17:38:48Z
category_4
category_7
category_8
category_12
category_46
category_64
category_132
portal_5
item_type_1
month_year_09_2026
<p>Vaccine-induced immunity is activated after infection but is overcome by <i>F. tularensis</i> FSC200.</p>
Pavlina Laskova (25138447)
Stanislava Porkertova (25138450)
Ondrej Ballek (11568544)
Lucie Balonova (1392496)
Jana Pavloskova (25138453)
Ales Neuwirth (13913055)
Ivona Pavkova (2339947)
Ivo Fabrik (1849519)
Pavol Lukac (25138456)
Luca Vannucci (14358999)
Dominik Filipp (1849615)
Jiri Stulik (151536)
Marek Link (151527)
Biochemistry
Medicine
Microbiology
Cell Biology
Immunology
Cancer
Infectious Diseases
sup >+</ sup
severe pneumonic form
induced immune responses
identified 57 mhc
div >< p
immunopeptidomics reveals conserved
protective immune response
peptide vaccine containing
fully virulent strains
ensure complete protection
cells following immunization
francisella tularensis </
tularensis </
using immunopeptidomics
based immunopeptidomics
vaccination studies
subsequent screening
significant role
potential role
least 80
ii peptides
human tularemia
highly expressed
fsc200 strain
five epitopes
epitopes proved
early control
dominant epitopes
characterizing antigen
cells playing
cell epitopes
bacterial proteome
23 different
<p>(A) Mice immunized with a peptide vaccine (PepVac) or treated with the same adjuvant but without peptides (control) were subcutaneously infected with 53 CFU of <i>F. tularensis</i> FSC200, and the vaccine’s protective efficacy was evaluated. (B) Changes in body weight of mice during infection. The body weight of the mice on the day of infection (day 0) was used as the baseline, and the graph shows the percentage changes relative to that day. Data from the four days prior to infection are also included. Values are presented as mean ± SD (one-sided). The data (days 1-5) were analyzed using a mixed-effects model with repeated measures, and multiple comparisons (PepVac versus control at individual time points) were corrected using Sidak’s post hoc test. An asterisk indicates a p-value < 0.05 for the interaction effect between time and treatment. (C) Survival curve for the vaccinated group (n = 8) and the control group of mice (n = 7). The dashed line indicates the median survival time for each group. The data were analyzed using the Kaplan-Meier method with the log-rank test (ns for p ≥ 0.05). Note: The unequal group sizes were due to the death of one mouse after administration of a booster dose as part of the immunization. The bacterial load in the spleen (D) and liver (E) of control and vaccinated mice (n = 5) was determined on day 4 postinfection. Data for individual mice and the corresponding mean values are shown. A two-tailed t-test was used to assess statistical significance. Asterisks indicate p-values < 0.05. This experiment was performed once.</p>
2026-09-29T17:54:09Z
Image
Figure
10.1371/journal.ppat.1014597.g007
https://figshare.com/articles/figure/_p_Vaccine-induced_immunity_is_activated_after_infection_but_is_overcome_by_i_F_tularensis_i_FSC200_p_/34026937
CC BY 4.0