2026-10-12T07:50:18Z
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2026-09-29T17:38:43Z
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<p><i>Francisella</i> MHC-II antigens and their relationship to the proteome.</p>
Pavlina Laskova (25138447)
Stanislava Porkertova (25138450)
Ondrej Ballek (11568544)
Lucie Balonova (1392496)
Jana Pavloskova (25138453)
Ales Neuwirth (13913055)
Ivona Pavkova (2339947)
Ivo Fabrik (1849519)
Pavol Lukac (25138456)
Luca Vannucci (14358999)
Dominik Filipp (1849615)
Jiri Stulik (151536)
Marek Link (151527)
Biochemistry
Medicine
Microbiology
Cell Biology
Immunology
Cancer
Infectious Diseases
sup >+</ sup
severe pneumonic form
induced immune responses
identified 57 mhc
div >< p
immunopeptidomics reveals conserved
protective immune response
peptide vaccine containing
fully virulent strains
ensure complete protection
cells following immunization
francisella tularensis </
tularensis </
using immunopeptidomics
based immunopeptidomics
vaccination studies
subsequent screening
significant role
potential role
least 80
ii peptides
human tularemia
highly expressed
fsc200 strain
five epitopes
epitopes proved
early control
dominant epitopes
characterizing antigen
cells playing
cell epitopes
bacterial proteome
23 different
<p>(A) COG annotation of <i>Francisella</i> LVS proteins entering the MHC class II pathway. Proteins associated with translation, cell wall, and lipid metabolism have emerged as important categories of proteins for antigen presentation. (B) Proteome analysis of <i>in vitro</i> cultured <i>Francisella</i> LVS. The 1,131 proteins identified at 1% FDR were ranked by their intensity (1,129 quantifiable) to express their relative abundance in the proteome. Most MHC-II antigens (squares) were highly expressed proteins. (C) A violin plot showing the intensity distribution for a group of 1,129 LVS proteins or 23 MHC-II proteins. The difference between the two groups was statistically significant (two-tailed Mann-Whitney test). The median, 25th, and 75th percentiles are shown. (D) Relative quantification of proteome changes induced by deletion of the <i>dsbA</i> gene in <i>Francisella</i> LVS. The ratios of mutant and wild-type proteins (n = 1,129) were plotted against the intensities of wild-type proteins. The localization of MHC-II proteins (squares) near the line indicating equal expression in both strains confirmed their unchanged availability for antigen presentation. In the otherwise unchanged LVSΔdsbA proteome, only a few proteins with altered expression were detected (diamonds). Proteins of interest are indicated by FTL numbers or gene names in all figures.</p>
2026-09-29T17:54:09Z
Image
Figure
10.1371/journal.ppat.1014597.g002
https://figshare.com/articles/figure/_p_i_Francisella_i_MHC-II_antigens_and_their_relationship_to_the_proteome_p_/34026922
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