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oai:figshare.com:article/34026909 2026-09-29T17:38:37Z category_4 category_8 category_13 category_14 category_873 category_46 category_734 category_106 category_132 portal_5 item_type_1 month_year_09_2026

<p>Viability of the RF spirochete <i>B. hermsii</i> in glycerol and MG.</p> Dan Drecktrah (36180) Laura S. Hall (12206815) Britney Cheff (25138443) Crystal Richards (12206818) Sandra J. Raffel (12240026) Michael Wulf (12206824) Ian T. Bailey (25138446) Frank Gherardini (40103) D. Scott Samuels (12206833) Biochemistry Microbiology Genetics Molecular Biology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Science Policy Infectious Diseases translation modification system reach high numbers amino acid residues activate carbonyl stress lyme disease spirochete carbonyl stress pathway relapsing fever spirochete div >< p lyme disease spirochetes enigmatic metabolite methylglyoxal burgdorferi mgsa </ carbonyl stress lyme disease relapsing fever mgsa </ burgdorferi </ mayonii </ hermsii </ borreliella </ borrelia </ bb0364 </ significantly reduces results identify relapse phase provide evidence previously believed phosphate imbalance peak level new post necessarily lethal murine infection mouse model metabolic regulation including glycerol dihydroxyacetone phosphate cellular carbohydrate <p><i>B. hermsii</i> were grown in normal growth medium before shifting to RPMI medium alone or containing increasing concentrations of glycerol (A) or MG (B) for 24 h at 35°C. Cells were plated in semi-solid BSK before (0 h) and after shifting to RPMI alone (0) or increasing concentrations of glycerol or MG. Values are the mean of three independent biological replicates expressed as % survival compared to cultures before shifting to RPMI. Error bars are the SE of the mean. Asterisks represent; * <i>P</i> = 0.004 and ** P < 0.001 as determined by one-way ANOVA with a Tukey’s <i>post-hoc</i> test.</p> 2026-09-29T17:55:00Z Image Figure 10.1371/journal.ppat.1014657.g010 https://figshare.com/articles/figure/_p_Viability_of_the_RF_spirochete_i_B_hermsii_i_in_glycerol_and_MG_p_/34026909 CC BY 4.0