2026-10-10T19:57:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34026903 2026-09-29T17:38:35Z category_4 category_8 category_13 category_14 category_873 category_46 category_734 category_106 category_132 portal_5 item_type_1 month_year_09_2026

Persistence of the mgsA mutant in ticks. Dan Drecktrah (36180) Laura S. Hall (12206815) Britney Cheff (25138443) Crystal Richards (12206818) Sandra J. Raffel (12240026) Michael Wulf (12206824) Ian T. Bailey (25138446) Frank Gherardini (40103) D. Scott Samuels (12206833) Biochemistry Microbiology Genetics Molecular Biology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Science Policy Infectious Diseases translation modification system reach high numbers amino acid residues activate carbonyl stress lyme disease spirochete carbonyl stress pathway relapsing fever spirochete div >< p lyme disease spirochetes enigmatic metabolite methylglyoxal burgdorferi mgsa </ carbonyl stress lyme disease relapsing fever mgsa </ burgdorferi </ mayonii </ hermsii </ borreliella </ borrelia </ bb0364 </ significantly reduces results identify relapse phase provide evidence previously believed phosphate imbalance peak level new post necessarily lethal murine infection mouse model metabolic regulation including glycerol dihydroxyacetone phosphate cellular carbohydrate B. burgdorferi strains were introduced to larval ticks by feeding on WT, ∆mgsA or mgsA+-infected mice. Acquisition and persistence of WT (black circles), ∆mgsA mutant (white circles), mgsA+ (dark gray circles) was assessed in ticks one week after larvae fed to repletion (A), following the molt to nymphs, nine weeks later (B) and one week after nymphs fed to repletion on naïve mice (C). DNA from infected ticks was isolated at each stage and B. burgdorferi load in ticks measured by TaqMan qPCR using primers/probe to flaB. Bars represent the mean. There was no significant difference between the means as determined by one-way ANOVA with a Tukey’s post-hoc test. 2026-09-29T17:55:00Z Image Figure 10.1371/journal.ppat.1014657.g008 https://figshare.com/articles/figure/_p_Persistence_of_the_i_mgsA_i_mutant_in_ticks_p_/34026903 CC BY 4.0