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<p>Regulation of the CSP and glycation by the stringent response and c-di-GMP signaling pathways.</p> Dan Drecktrah (36180) Laura S. Hall (12206815) Britney Cheff (25138443) Crystal Richards (12206818) Sandra J. Raffel (12240026) Michael Wulf (12206824) Ian T. Bailey (25138446) Frank Gherardini (40103) D. Scott Samuels (12206833) Biochemistry Microbiology Genetics Molecular Biology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Science Policy Infectious Diseases translation modification system reach high numbers amino acid residues activate carbonyl stress lyme disease spirochete carbonyl stress pathway relapsing fever spirochete div >< p lyme disease spirochetes enigmatic metabolite methylglyoxal burgdorferi mgsa </ carbonyl stress lyme disease relapsing fever mgsa </ burgdorferi </ mayonii </ hermsii </ borreliella </ borrelia </ bb0364 </ significantly reduces results identify relapse phase provide evidence previously believed phosphate imbalance peak level new post necessarily lethal murine infection mouse model metabolic regulation including glycerol dihydroxyacetone phosphate cellular carbohydrate <p>Wild-type (WT), <i>rel</i>_Bbu mutant (∆<i>rel</i>_Bbu) (A), <i>dksA</i> mutant (∆<i>dksA</i>) (B), <i>rrp1</i> mutant (∆<i>rrp1</i>) (C), or <i>plzA</i> mutant (∆<i>plzA</i>) (D) and respective complemented strains were grown to ~7 × 10⁷ cell ml⁻¹ in normal BSK + RS growth medium and whole cell lysates collected or shifted to nutrient stress medium containing glycerol (RPMI + glycerol) or neopeptone (RPMI + neopeptone) for 24 h before collecting cell lysates. Samples were separated by SDS-PAGE and analyzed by immunoblot using antibodies against MG (to recognize glycated proteins), MgsA and FlaB.</p> 2026-09-29T17:55:00Z Image Figure 10.1371/journal.ppat.1014657.g007 https://figshare.com/articles/figure/_p_Regulation_of_the_CSP_and_glycation_by_the_stringent_response_and_c-di-GMP_signaling_pathways_p_/34026900 CC BY 4.0