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<p>Sph deficiency attenuates <i>S. aureus</i>-induced mastitis and ferroptotic tissue injury in mice.</p>
Yunfei Wei (9412265)
Xinyu Zhao (102214)
Ziyi Yin (4117480)
Yanmin Zhang (391964)
Yaxin Zhai (2562517)
Baihe Zhan (25138380)
Yeling Wang (371383)
Jiakang He (16829817)
Jianfeng Wang (232744)
Xuming Deng (158166)
Lei Xu (78676)
Haihua Feng (198813)
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Science Policy
Infectious Diseases
Virology
mitochondrial contact site
dysregulated inflammatory response
disrupt mammary tissues
potential therapeutic target
key virulence determinant
mastitis remain elusive
dependent ferroptosis contribute
>- induced mastitis
>- induced ferroptosis
sting signaling pathway
mediated mtdna release
cgas ), leading
staphylococcus aureus </
promote gpx4 degradation
aureus </
virulence factors
sting axis
potential role
lysosome pathway
underlying mechanisms
thereby eliciting
sphingomyelin phosphodiesterase
organizing system
micos ).
major pathogen
diverse arsenal
cyclic gmp
core component
amp synthase
>; however
<p>Mice were intramammarily inoculated through mammary gland with 5 × 10⁶ CFU of <i>S. aureus</i> WT, Δ<i>sph</i>, or Δ<i>sph::sph</i> strains. Infected mice received deferiprone (50 mg/kg) or vehicle by oral gavage every 8 h for 48 h (n = 6 per group). <b>(A)</b> Gross morphology of mammary glands. <b>(B)</b> Representative H&E-stained sections of mammary tissues. Scale bars, 400 μm. <b>(C)</b> Bacterial burdens in mammary tissues. <b>(D, E)</b> TNF-α <b>(</b><b>D</b><b>)</b> and IL-6 <b>(</b><b>E</b><b>)</b> levels in mammary tissue homogenates. <b>(F)</b> GSH levels in mammary tissues. <b>(G)</b> MDA levels in mammary tissues. <b>(H)</b> MPO activity in mammary tissues. <b>(I)</b> Representative immunohistochemical staining of GPX4 in mammary tissues. Scale bars, 400 μm. Data are presented as mean ± SD. *<i>P</i> < 0.05; **<i>P</i> < 0.01; ns, not significant.</p>
2026-09-29T18:02:15Z
Image
Figure
10.1371/journal.ppat.1014644.g007
https://figshare.com/articles/figure/_p_Sph_deficiency_attenuates_i_S_aureus_i_-induced_mastitis_and_ferroptotic_tissue_injury_in_mice_p_/34026606
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