2026-10-12T15:37:25Z
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2026-09-29T17:32:43Z
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<p>The <i>sph</i> deletion mutant attenuates ferroptosis-associated phenotypes and bacterial virulence.</p>
Yunfei Wei (9412265)
Xinyu Zhao (102214)
Ziyi Yin (4117480)
Yanmin Zhang (391964)
Yaxin Zhai (2562517)
Baihe Zhan (25138380)
Yeling Wang (371383)
Jiakang He (16829817)
Jianfeng Wang (232744)
Xuming Deng (158166)
Lei Xu (78676)
Haihua Feng (198813)
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Science Policy
Infectious Diseases
Virology
mitochondrial contact site
dysregulated inflammatory response
disrupt mammary tissues
potential therapeutic target
key virulence determinant
mastitis remain elusive
dependent ferroptosis contribute
>- induced mastitis
>- induced ferroptosis
sting signaling pathway
mediated mtdna release
cgas ), leading
staphylococcus aureus </
promote gpx4 degradation
aureus </
virulence factors
sting axis
potential role
lysosome pathway
underlying mechanisms
thereby eliciting
sphingomyelin phosphodiesterase
organizing system
micos ).
major pathogen
diverse arsenal
cyclic gmp
core component
amp synthase
>; however
<p><b>(A)</b> Growth curves of <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> strains (n = 3). <b>(B)</b> Biofilm formation of <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> strains (n = 3). <b>(C)</b> LDH release from MAC-T cells infected with <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> strains (n = 5). <b>(D)</b> Survival rates of <i>Galleria mellonella</i> infected with <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> strains (n = 9). <b>(E, F)</b> Representative immunoblot and quantitative analysis of GPX4 expression in MAC-T cells infected with <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> strains for 6 h (n = 3). <b>(G-J)</b> Representative fluorescence images and <b>(</b><b>K-N</b><b>)</b> corresponding quantitative analyses of intracellular ROS (DCFH-DA; <b>G</b>, <b>K)</b>, Fe<sup>2+</sup> accumulation <b>(H, L)</b>, lipid peroxidation (C11 BODIPY 581/591; <b>I</b>, <b>M)</b>, and mitochondrial membrane potential (JC-10; <b>J, N</b>) in MAC-T cells infected with the indicated <i>S. aureus</i> strains (n = 3). Scale bars, 200 μm or 100 μm. Data are presented as mean ± SD. *<i>P</i> < 0.05; **<i>P</i> < 0.01; ns, not significant.</p>
2026-09-29T18:02:15Z
Image
Figure
10.1371/journal.ppat.1014644.g002
https://figshare.com/articles/figure/_p_The_i_sph_i_deletion_mutant_attenuates_ferroptosis-associated_phenotypes_and_bacterial_virulence_p_/34026591
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