2026-10-13T22:55:08Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34026555
2026-09-29T17:32:31Z
category_4
category_8
category_12
category_13
category_14
category_272
category_46
category_734
category_106
category_132
category_134
portal_5
item_type_1
month_year_09_2026
<p>Expression, purification, and functional validation of recombinant Sph protein. (A) PCR amplification of the <i>sph</i> coding sequence from <i>S. aureus</i> ATCC25923. (B) PCR verification of <i>sph</i> in <i>E. coli</i> DH5α (pET-28a(+)-<i>sph</i>)<i>.</i> (C) Double-digestion analysis confirming correct construction of pET-28a(+)-<i>sph</i> in <i>E. coli</i> DH5α. (D) Imidazole-gradient elution of recombinant Sph protein following affinity purification. (E) Validation of the hemolytic activity of recombinant Sph protein. (F) Inhibitory effect of GW4869 on the hemolytic activity of recombinant Sph. Data are presented as mean ± SD.</p>
Yunfei Wei (9412265)
Xinyu Zhao (102214)
Ziyi Yin (4117480)
Yanmin Zhang (391964)
Yaxin Zhai (2562517)
Baihe Zhan (25138380)
Yeling Wang (371383)
Jiakang He (16829817)
Jianfeng Wang (232744)
Xuming Deng (158166)
Lei Xu (78676)
Haihua Feng (198813)
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Science Policy
Infectious Diseases
Virology
mitochondrial contact site
dysregulated inflammatory response
disrupt mammary tissues
potential therapeutic target
key virulence determinant
mastitis remain elusive
dependent ferroptosis contribute
>- induced mastitis
>- induced ferroptosis
sting signaling pathway
mediated mtdna release
cgas ), leading
staphylococcus aureus </
promote gpx4 degradation
aureus </
virulence factors
sting axis
potential role
lysosome pathway
underlying mechanisms
thereby eliciting
sphingomyelin phosphodiesterase
organizing system
micos ).
major pathogen
diverse arsenal
cyclic gmp
core component
amp synthase
>; however
<p>Expression, purification, and functional validation of recombinant Sph protein. (A) PCR amplification of the <i>sph</i> coding sequence from <i>S. aureus</i> ATCC25923. (B) PCR verification of <i>sph</i> in <i>E. coli</i> DH5α (pET-28a(+)-<i>sph</i>)<i>.</i> (C) Double-digestion analysis confirming correct construction of pET-28a(+)-<i>sph</i> in <i>E. coli</i> DH5α. (D) Imidazole-gradient elution of recombinant Sph protein following affinity purification. (E) Validation of the hemolytic activity of recombinant Sph protein. (F) Inhibitory effect of GW4869 on the hemolytic activity of recombinant Sph. Data are presented as mean ± SD.</p>
2026-09-29T18:02:15Z
Image
Figure
10.1371/journal.ppat.1014644.s002
https://figshare.com/articles/figure/_p_XXX_p_/34026555
CC BY 4.0