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oai:figshare.com:article/34026466
2026-09-29T17:31:34Z
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<p>The intestinal function of NHR-68 is required for pathogen avoidance behavior.</p>
Jie Ren (176083)
Yu Sang (2551267)
Young-Mo Kim (287055)
Ernesto S. Nakayasu (156378)
Alejandro Aballay (51596)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Chemical Sciences not elsewhere classified
Ecology
Science Policy
Immunology
Developmental Biology
Mental Health
Computational Biology
polyunsaturated fatty acid
div >< p
caenorhabditis elegans </
brain transcriptional circuit
enhances pathogen avoidance
68 suppresses activation
la restores avoidance
avoidance circuit
dependent pathogen
3 </
2 </
p38 mapk
neuron communication
la synthesis
immune outputs
foxo pathways
findings identify
elevates la
behavioral immunity
68 acts
<p><b>(A)</b><i>P. aeruginosa</i> occupancy index of wild-type animals fed empty vector control or <i>nhr-68</i> RNAi at 8 h and 12 h. <b>(B)</b> <i>P. aeruginosa</i> occupancy index of MGH171 animals fed empty vector control or <i>nhr-68</i> RNAi at 8 h and 12 h. <b>(C)</b> <i>P. aeruginosa</i> occupancy index of MAH677 animals fed empty vector control or <i>nhr-68</i> RNAi at 8 hr and 12 hr. <b>(D)</b> <i>P. aeruginosa</i> occupancy index for WT, <i>nhr-68(gk708)</i> and <i>nhr-68(gk708);vha-6p::nhr-68</i> animals at 8 h and 12 h after seeding. The occupancy index was calculated as (<i>N</i><sub>on</sub> lawn/<i>N</i><sub>total</sub>). Data are presented as mean ± SD from three independent experiments (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). <b>(E)</b> Survival of wild-type animals fed empty vector control or <i>nhr-68</i> RNAi on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.24 and 2.89 days, respectively. Empty vector vs. <i>nhr-68</i> RNAi, <i>p</i> < 0.0001. <b>(F)</b> Survival of MGH171 animals fed empty vector control or <i>nhr-68</i> RNAi on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.23 and 3.02 days, respectively. Empty vector vs. <i>nhr-68</i> RNAi, <i>p</i> < 0.0001. <b>(G)</b> Survival of MAH677 animals fed empty vector control or <i>nhr-68</i> RNAi on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.27 and 2.28 days, respectively. Empty vector vs. <i>nhr-68</i> RNAi, <i>p</i> = ns. <b>(H)</b> Survival of wild-type N2, <i>nhr-68(gk708)</i>, or <i>nhr-68(gk708);vha-6p::nhr-68</i> animals on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.17, 2.81, and 2.40 days, respectively. Wild-type vs. <i>nhr-68(gk708)</i>, <i>p</i> < 0.0001; Wild-type vs. <i>nhr-68(gk708);vha-6p::nhr-68</i>, <i>p</i> = 0.0145; <i>nhr-68(gk708)</i> vs. <i>nhr-68(gk708);vha-6p::nhr-68</i>, <i>p</i> = 0.0017. Data are presented from three independent experiments, with 90 animals per condition per experiment (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). The Kaplan-Meier method was used to calculate the survival fractions, and statistical significance between survival curves was determined using the log-rank test. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004026#pbio.3004026.s009" target="_blank">S1 Data</a>.</p>
2026-09-29T17:57:38Z
Image
Figure
10.1371/journal.pbio.3004026.g006
https://figshare.com/articles/figure/_p_The_intestinal_function_of_NHR-68_is_required_for_pathogen_avoidance_behavior_p_/34026466
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