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2026-09-29T17:31:30Z
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<p>NHR-68 inhibits immune pathways.</p>
Jie Ren (176083)
Yu Sang (2551267)
Young-Mo Kim (287055)
Ernesto S. Nakayasu (156378)
Alejandro Aballay (51596)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Chemical Sciences not elsewhere classified
Ecology
Science Policy
Immunology
Developmental Biology
Mental Health
Computational Biology
polyunsaturated fatty acid
div >< p
caenorhabditis elegans </
brain transcriptional circuit
enhances pathogen avoidance
68 suppresses activation
la restores avoidance
avoidance circuit
dependent pathogen
3 </
2 </
p38 mapk
neuron communication
la synthesis
immune outputs
foxo pathways
findings identify
elevates la
behavioral immunity
68 acts
<p><b>(A)</b> Gene Ontology analysis of genes upregulated in <i>nhr-68(gk708)</i> vs. WT animals after 12 h infection with <i>P. aeruginosa</i>. The results were filtered to include only terms significantly enriched in <i>nhr-68(gk708)</i> with a <i>q</i> value <0.1. <b>(B)</b> The representation factors of the identified upregulated genes controlled by the PMK-1, DAF-16, SKN-1, and ELT-2 pathways. <b>(C)</b> qRT–PCR analysis of PMK-1- and DAF-16-dependent genes in <i>nhr-68(gk708)</i> compared with WT animals. (<i>N</i> = 3 biological replicates). <b>(D)</b> Survival of wild-type and <i>nhr-68(gk708)</i> animals fed empty vector control or <i>pmk-1</i> RNAi followed by exposure to <i>P. aeruginosa</i>. Mean survival times were 2.40 and 3.01 days for empty vector control RNAi and 1.88 and 1.92 days for <i>pmk-1</i> RNAi, respectively. Wild-type vs. <i>nhr-68(gk708)</i>, <i>p</i> < 0.0001 and <i>p</i> = 0.1121 for empty vector control and <i>pmk-1</i> RNAi, respectively. Data are presented from three independent experiments, with 90 animals per condition per experiment (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). The Kaplan–Meier method was used to calculate the survival fractions, and statistical significance between survival curves was determined using the log-rank test. <b><i>(E)</i></b> Survival of wild-type and <i>nhr-68(gk708)</i> animals fed empty vector control or <i>daf-16</i> RNAi followed by exposure to <i>P. aeruginosa</i>. Mean survival times were 2.40 and 3.01 days for empty vector control RNAi and 2.14 and 2.43 days for <i>daf-16</i> RNAi, respectively. Wild-type vs. <i>nhr-68(gk708)</i>, <i>p</i> < 0.0001 and <i>p</i> = 0.0130 for empty vector control and <i>daf-16</i> RNAi, respectively. Data are presented from three independent experiments, with 90 animals per condition per experiment (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). The Kaplan–Meier method was used to calculate the survival fractions, and statistical significance between survival curves was determined using the log-rank test. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004026#pbio.3004026.s009" target="_blank">S1 Data</a>.</p>
2026-09-29T17:57:38Z
Image
Figure
10.1371/journal.pbio.3004026.g003
https://figshare.com/articles/figure/_p_NHR-68_inhibits_immune_pathways_p_/34026454
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