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2026-09-29T17:31:28Z
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<p>NHR-10 and NHR-68 inhibit innate immune response.</p>
Jie Ren (176083)
Yu Sang (2551267)
Young-Mo Kim (287055)
Ernesto S. Nakayasu (156378)
Alejandro Aballay (51596)
Biochemistry
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Chemical Sciences not elsewhere classified
Ecology
Science Policy
Immunology
Developmental Biology
Mental Health
Computational Biology
polyunsaturated fatty acid
div >< p
caenorhabditis elegans </
brain transcriptional circuit
enhances pathogen avoidance
68 suppresses activation
la restores avoidance
avoidance circuit
dependent pathogen
3 </
2 </
p38 mapk
neuron communication
la synthesis
immune outputs
foxo pathways
findings identify
elevates la
behavioral immunity
68 acts
<p><b>(A)</b> Representative fluorescence micrographs of <i>clec-60p::gfp</i> animals grown on <i>E. coli</i> HT115 carrying empty vector or RNAi against <i>nhr-77</i>, <i>nhr-10</i>, <i>nhr-130</i>, <i>nhr-222</i>, <i>nhr-162</i>, <i>nhr-42</i>, <i>nhr-140</i>, <i>nhr-68</i>, <i>nhr-54</i>, or <i>nhr-10</i> and <i>nhr-68</i> (<i>n</i> = 8 animals per condition per experiment; <i>N</i> = 3 independent experiments). Fluorescence images were captured with 100-ms exposures. Scale bar, 200 µm. <b>(B)</b> qRT–PCR analysis of the immune gene <i>clec-60</i> in wild-type, <i>nhr-10(tm4695)</i>, <i>nhr-68(gk708)</i> and <i>nhr-10(tm4695)</i>; <i>nhr-68(gk708)</i> animals fed on <i>E. coli</i>. Data are presented as the mean ± SD from three independent biological replicates (<i>N</i> = 3; <i>n</i> = 200 animals per condition per biological replicate). ***<i>p</i> < 0.001, one-way ANOVA with Tukey’s multiple-comparison test. <b>(C)</b> Survival of WT, <i>nhr-10(tm4695)</i>, <i>nhr-68(gk708)</i> or <i>nhr-10(tm4695)</i>;<i>nhr-68(gk708)</i> animals grown on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.06, 2.36, 2.82, and 3.13 days, respectively. Data are presented from three independent experiments, with 90 animals per condition per experiment (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). The Kaplan–Meier method was used to calculate the survival fractions, and statistical significance between survival curves was determined using the log-rank test. WT vs. <i>nhr-10(tm4695)</i>, <i>p</i> < 0.01; WT vs. <i>nhr-68(gk708)</i>, <i>p</i> < 0.001; WT vs. <i>nhr-10(tm4695)</i>; <i>nhr-68(gk708)</i>, <i>p</i> < 0.001; <i>nhr-10(tm4695)</i> vs. <i>nhr-10(tm4695)</i>; <i>nhr-68(gk708)</i>, <i>p</i> < 0.001; <i>nhr-68(gk708)</i> vs. <i>nhr-10(tm4695)</i>; <i>nhr-68(gk708)</i>, <i>p</i> = 0.0435. <b>(D)</b> Occupancy index for WT, <i>nhr-10(tm4695)</i>, <i>nhr-68(gk708)</i> or <i>nhr-10(tm4695)</i>;<i>nhr-68(gk708)</i> animals on <i>P. aeruginosa</i> at 8 h and 12 h. The occupancy index was calculated as (<i>N</i><sub>on</sub> lawn/<i>N</i><sub>total</sub>). Data are presented as mean ± SD from three independent experiments (<i>N</i> = 3). The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004026#pbio.3004026.s009" target="_blank">S1 Data</a>.</p>
2026-09-29T17:57:38Z
Image
Figure
10.1371/journal.pbio.3004026.g001
https://figshare.com/articles/figure/_p_NHR-10_and_NHR-68_inhibit_innate_immune_response_p_/34026448
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