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oai:figshare.com:article/34026346 2026-09-29T17:30:02Z category_1 category_4 category_12 category_14 category_15 category_21 category_24 category_45 category_61 category_64 category_132 category_133 portal_5 item_type_6 month_year_09_2026

<p>Effect of labeling and experimental system on the G_s protein mobility.</p> Ondřej Kuchynka (25138312) Andrei Kovalchuk (25138315) Mia Nußbaumer (25138318) Ekaterina Sviridova (25138321) Tomáš Fessl (25138324) Alexey Bondar (665062) Biophysics Biochemistry Cell Biology Molecular Biology Neuroscience Biotechnology Evolutionary Biology Sociology Developmental Biology Cancer Infectious Diseases Plant Biology remain poorly understood physical signals downstream many biophysical aspects key signal transducers findings identify subtype div >< p amplify extracellular chemical g &# 945 specific mobility differences subunits exhibit subtype heterotrimeric g proteins including membrane mobility g proteins membrane mobility g protein plasma membrane xlink "> using single subunit plays signaling dynamics results indicate molecule imaging major role live cells inner leaflet eukaryotic cells different subtypes coupled receptors </ sub <p>A) Median diffusion coefficients of G_s heterotrimers observed using overexpression of Gα_s and Gβ₁ under the CMV promoter, and underexpression of Gγ₂-HaloTag under the minP promoter (G_s: <i>n</i> = 15, observed range = 0.23–0.46 μm²/sec), as well as through overexpression of Gγ₂ and Gβ₁ under the CMV promoter, and underexpression of Gα_s-HaloTag under the minP promoter (G_sHaloTag; <i>n</i> = 15, observed range = 0.19–39 μm²/sec). Additionally, diffusion coefficient medians of Gγ₂-labeled heterotrimers containing the Gα_s-ΔCt2 mutant, missing two C-terminal amino acids, are shown (G_s-ΔCt2; <i>n</i> = 10, observed range = 0.08–0.58 μm²/sec). B) Diffusion coefficient medians of the G_s protein (<i>n</i> = 15, observed range = 0.23–0.46 μm²/sec) coexpressed with the vasopressin 2 receptor (G_s + V2R, untreated; <i>n</i> = 11, observed range = 0.1–0.64 μm²/sec) and treated with arginine vasopressin 1μM (G_s + V2R+AVP, separated into time intervals post-treatment; 0–15 min: <i>n</i> = 4, observed range = 0.09–0.16 μm²/sec; 16–30 min: <i>n</i> = 5, observed range = 0.13–0.51 μm²/sec; 31–45 min: <i>n</i> = 6, observed range = 0.08–0.44 μm²/sec). The boxes indicate the median and the interquartile range. Due to low sample size, no statistical inference was performed on the medians shown in A and B. C) Diffusion coefficient medians of G_s expressed in CHO-K1 cells (G_s) and HEK-293 cells (G_s(HEK-293)). Statistical analysis results are indicated above (<i>α</i> = 0.05, <i>p</i> < 0.0001, <i>U</i> = 12, <i>r</i>_<i>rb</i> = 0.89).</p> <p>(PDF)</p> 2026-09-29T17:29:54Z Text Journal contribution 10.1371/journal.pone.0359304.s006 https://figshare.com/articles/journal_contribution/_p_Effect_of_labeling_and_experimental_system_on_the_G_sub_s_sub_protein_mobility_p_/34026346 CC BY 4.0