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oai:figshare.com:article/34026301 2026-09-29T17:29:44Z category_8 category_13 category_14 category_272 category_39 category_46 category_734 category_106 category_122 category_132 category_133 category_134 portal_5 item_type_1 month_year_09_2026

<p>Grass carp NHRE3 inhibits ISG15-mediated antiviral function.</p> Juhong Xie (25138306) Jiayi Lei (7853165) Qinxue Zhu (25138309) Bin Gui (542725) Yongming Li (584924) Yaping Wang (289641) Rong Huang (298496) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Science Policy Mental Health Infectious Diseases Plant Biology Virology specific ubiquitin ligase significant scientific importance rare minnow (< innate immune regulation immunological arms race grass carp reovirus grass carp (< achieve immune escape gobiocypris rarus </ ctenopharyngodon idella </ virus promotes degradation stimulated gene 15 gchd ), caused >) hemorrhagic diseases viruses exploit ubiquitination host interferon response div >< p ubiquitination plays hemorrhagic disease gene encodes degradation via nhre3 </ gcrv ), viral evasion translational value thereby exerting taken together serious threat random mutagenesis proteasome pathway mechanism unique may represent linked polyubiquitination diverse form central role catalyzing k48 antiviral mechanism ancient origins <p>(A) CIK cells co-expressing ISG15 and NHRE3 were infected with GCRV. CPE was assessed using crystal violet staining 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls. (B) GCO cells co-expressing ISG15 and NHRE3 were infected with GCRV. The expression level of the GCRV VP5 protein was measured via western blot 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls. (C) GCO cells co-expressing ISG15 and NHRE3 were infected with GCRV. The expression levels of the GCRV S6, S10, and NS1 segments were assessed using qPCR 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls. (D) GCO cells co-expressing ISG15 and different NHRE3 truncation mutants were infected with GCRV. The expression levels of the GCRV S6, S10, and NS1 segments were assessed using qPCR 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls. (E) GCO cells co-expressing ISG15 and different NHRE3 truncation mutants were infected with GCRV. The expression level of the GCRV VP5 protein was measured via western blot 24 h post-infection. Cells transfected with the empty vector and those transfected with NHRE3 alone served as controls. All experiments were repeated for at least three times with similar results.</p> 2026-09-29T17:29:22Z Image Figure 10.1371/journal.ppat.1014544.g006 https://figshare.com/articles/figure/_p_Grass_carp_NHRE3_inhibits_ISG15-mediated_antiviral_function_p_/34026301 CC BY 4.0