2026-10-13T20:40:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34026298 2026-09-29T17:29:43Z category_8 category_13 category_14 category_272 category_39 category_46 category_734 category_106 category_122 category_132 category_133 category_134 portal_5 item_type_1 month_year_09_2026

<p>Grass carp NHRE3 degrades ISG15 via the K48-linked ubiquitination–proteasome pathway.</p> Juhong Xie (25138306) Jiayi Lei (7853165) Qinxue Zhu (25138309) Bin Gui (542725) Yongming Li (584924) Yaping Wang (289641) Rong Huang (298496) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Science Policy Mental Health Infectious Diseases Plant Biology Virology specific ubiquitin ligase significant scientific importance rare minnow (< innate immune regulation immunological arms race grass carp reovirus grass carp (< achieve immune escape gobiocypris rarus </ ctenopharyngodon idella </ virus promotes degradation stimulated gene 15 gchd ), caused >) hemorrhagic diseases viruses exploit ubiquitination host interferon response div >< p ubiquitination plays hemorrhagic disease gene encodes degradation via nhre3 </ gcrv ), viral evasion translational value thereby exerting taken together serious threat random mutagenesis proteasome pathway mechanism unique may represent linked polyubiquitination diverse form central role catalyzing k48 antiviral mechanism ancient origins <p>(A) Western blot analysis of NHRE3 and ISG15 in CIK cells following GCRV infection, showing ISG degradation. (B) After overexpressing NHRE3, the CIK cells were infected with GCRV, WB was used to detect ISG15 protein expression after 24h, with the uninfected and empty vector overexpression groups serving as controls. (C) After overexpressing <i>nhre3</i>, the CIK cells were infected with GCRV, qPCR was used to detect isg15 gene expression after 24h, with the uninfected and empty vector overexpression groups serving as controls. (D) 293T cells were co-transfected with 0, 1, or 2 μg of pcDNA-NHRE3-HA and pCMV-ISG15-MYC. After 18 h, the cells were treated with MG-132 (20 μM) for 6 h, and ISG15 degradation was assessed via western blot. (E) 293T cells were co-transfected with pcDNA-NHRE3-HA, pCMV-ISG15-MYC, and pCMV-Ub-His. ISG15 ubiquitination was assessed using western blot. A control group without pcDNA-NHRE3-HA transfection was included. (F) 293T cells were co-transfected with pcDNA-NHRE3-HA and pCMV-ISG15-MYC in the presence of His-tagged wild-type ubiquitin (WT) or ubiquitin mutants, including K11-only (K11O), K48-only (K48O), and K63-only (K63O). The cells were treated with MG-132 (20 μM) for 6 h, and ISG15 ubiquitination was subsequently analyzed via western blot. A control group without pcDNA-NHRE3-HA transfection was included. All experiments were repeated for at least three times with similar results.</p> 2026-09-29T17:29:22Z Image Figure 10.1371/journal.ppat.1014544.g005 https://figshare.com/articles/figure/_p_Grass_carp_NHRE3_degrades_ISG15_via_the_K48-linked_ubiquitination_proteasome_pathway_p_/34026298 CC BY 4.0