2026-10-14T09:25:17Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34026295 2026-09-29T17:29:41Z category_8 category_13 category_14 category_272 category_39 category_46 category_734 category_106 category_122 category_132 category_133 category_134 portal_5 item_type_1 month_year_09_2026

<p>Grass carp NHRE3 interacts with ISG15.</p> Juhong Xie (25138306) Jiayi Lei (7853165) Qinxue Zhu (25138309) Bin Gui (542725) Yongming Li (584924) Yaping Wang (289641) Rong Huang (298496) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Science Policy Mental Health Infectious Diseases Plant Biology Virology specific ubiquitin ligase significant scientific importance rare minnow (< innate immune regulation immunological arms race grass carp reovirus grass carp (< achieve immune escape gobiocypris rarus </ ctenopharyngodon idella </ virus promotes degradation stimulated gene 15 gchd ), caused >) hemorrhagic diseases viruses exploit ubiquitination host interferon response div >< p ubiquitination plays hemorrhagic disease gene encodes degradation via nhre3 </ gcrv ), viral evasion translational value thereby exerting taken together serious threat random mutagenesis proteasome pathway mechanism unique may represent linked polyubiquitination diverse form central role catalyzing k48 antiviral mechanism ancient origins <p>(A) Non-specific proteins identified via immunoprecipitation–mass spectrometry (IP–MS) in the control group (NHRE3 overexpression without viral challenge). (B) Candidate interacting proteins identified via IP–MS in the experimental group (NHRE3 overexpression with viral challenge). (C) Venn diagram of proteins identified with IP–MS in the control and experimental groups. After excluding 23 non-specific proteins found in the control group, six candidate interacting proteins were identified in the experimental group. (D) Annotation information for the six candidate interacting proteins. (E) Co-immunoprecipitation (Co-IP) was performed 24 h after co-transfection of pcDNA-NHRE3-HA and pCMV-ISG15-MYC in 293T cells. A distinct band was observed in the IP fraction corresponding to the interaction between NHRE3 and ISG15. (F) Colocalization analysis was performed 24 h after co-transfection of pGFP-NHRE3 and pmCherry-ISG15 in CIK cells. Confocal microscopy revealed cytoplasmic colocalization, which was confirmed by analysis using the ImageJ Colocalization Finder plugin, yielding a Pearson correlation coefficient of 0.57. (G) Domain structure of NHRE3 and construction of truncation mutants. (H) Co-IP was performed 24 h after co-transfection of NHRE3_N and NHRE3_C truncation mutants with pCMV-ISG15-MYC in 293T cells. A distinct band was observed in the IP fraction corresponding to the interaction between the NHRE3_C mutant and ISG15. All experiments were repeated for at least three times with similar results.</p> 2026-09-29T17:29:22Z Image Figure 10.1371/journal.ppat.1014544.g004 https://figshare.com/articles/figure/_p_Grass_carp_NHRE3_interacts_with_ISG15_p_/34026295 CC BY 4.0