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oai:figshare.com:article/34026292 2026-09-29T17:29:40Z category_8 category_13 category_14 category_272 category_39 category_46 category_734 category_106 category_122 category_132 category_133 category_134 portal_5 item_type_1 month_year_09_2026

The nhre3 gene responds to and promotes GCRV infection. Juhong Xie (25138306) Jiayi Lei (7853165) Qinxue Zhu (25138309) Bin Gui (542725) Yongming Li (584924) Yaping Wang (289641) Rong Huang (298496) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Science Policy Mental Health Infectious Diseases Plant Biology Virology specific ubiquitin ligase significant scientific importance rare minnow (< innate immune regulation immunological arms race grass carp reovirus grass carp (< achieve immune escape gobiocypris rarus </ ctenopharyngodon idella </ virus promotes degradation stimulated gene 15 gchd ), caused >) hemorrhagic diseases viruses exploit ubiquitination host interferon response div >< p ubiquitination plays hemorrhagic disease gene encodes degradation via nhre3 </ gcrv ), viral evasion translational value thereby exerting taken together serious threat random mutagenesis proteasome pathway mechanism unique may represent linked polyubiquitination diverse form central role catalyzing k48 antiviral mechanism ancient origins (A) qPCR analysis of nhre3 gene expression in the gill, hepatopancreas, kidney, and intestine of rare minnow on days 0, 3, and 7 post-GCRV infection. (B) qPCR analysis of nhre3 gene expression in the gill, hepatopancreas, kidney, and intestine of grass carp on days 0, 3, and 7 post-GCRV infection. (C) qPCR analysis of nhre3 gene expression in CIK cells at 0, 6, 12, 24, and 36 h post-GCRV infection. (D) Western blot analysis of NHRE3 protein and GCRV VP5 protein levels in CIK cells at 0, 6, 12, 24, and 36 h post-GCRV infection. (E) qPCR analysis of ifn1, isg15, and nhre3 gene expression levels in CIK cells treated with the activator Vadimezan (100μg/ml) after 24h, DMSO treatment as the control group. (F) CIK cells overexpressing nhre3 for 24 h were subsequently challenged with GCRV. The expression level of the GCRV S10 segment was measured using qPCR at 8, 16, and 24 h post-challenge. Cells transfected with an empty vector served as the control group. (G) CIK cells overexpressing nhre3 for 24 h were subsequently challenged with GCRV. The expression level of the GCRV NS1 segment was measured using qPCR at 8, 16, and 24 h post-challenge. Cells transfected with an empty vector served as the control group. (H) CIK cells were transfected with 0, 1, or 2 μg of nhre3 overexpression plasmid for 24 h and subsequently challenged with GCRV. The expression levels of GCRV VP5 and NHRE3 proteins were measured via western blot at 24 h post-challenge. (I) CIK cells overexpressing nhre3 for 24 h were subsequently challenged with GCRV. Cytopathic effects (CPE) were assessed using crystal violet staining at 24 h post-challenge. All experiments were repeated for at least three times with similar results. 2026-09-29T17:29:22Z Image Figure 10.1371/journal.ppat.1014544.g003 https://figshare.com/articles/figure/_p_The_i_nhre3_i_gene_responds_to_and_promotes_GCRV_infection_p_/34026292 CC BY 4.0