2026-10-09T22:56:53Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34026289 2026-09-29T17:29:39Z category_8 category_13 category_14 category_272 category_39 category_46 category_734 category_106 category_122 category_132 category_133 category_134 portal_5 item_type_1 month_year_09_2026

A nhre3 homozygous mutant of rare minnow exhibits resistance to GCRV. Juhong Xie (25138306) Jiayi Lei (7853165) Qinxue Zhu (25138309) Bin Gui (542725) Yongming Li (584924) Yaping Wang (289641) Rong Huang (298496) Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Ecology Immunology Biological Sciences not elsewhere classified Science Policy Mental Health Infectious Diseases Plant Biology Virology specific ubiquitin ligase significant scientific importance rare minnow (< innate immune regulation immunological arms race grass carp reovirus grass carp (< achieve immune escape gobiocypris rarus </ ctenopharyngodon idella </ virus promotes degradation stimulated gene 15 gchd ), caused >) hemorrhagic diseases viruses exploit ubiquitination host interferon response div >< p ubiquitination plays hemorrhagic disease gene encodes degradation via nhre3 </ gcrv ), viral evasion translational value thereby exerting taken together serious threat random mutagenesis proteasome pathway mechanism unique may represent linked polyubiquitination diverse form central role catalyzing k48 antiviral mechanism ancient origins (A) Mutation type in the homozygous nhre3 knockout mutant of rare minnow. Compared with the wild type, the mutant harbors a 12 bp deletion and a 13 bp insertion within exon 6. (B) Total RNA was extracted from wild-type and mutant larvae, and the expression level of nhre3 was assessed using qPCR. Expression of nhre3 in the mutant was significantly reduced compared with that in the wild type. (C) Survival curves of larvae following viral challenge. Mutant larvae exhibited prolonged survival and a reduced mortality rate compared with those in the wild type. (D) Survival curves of adult fish following viral challenge. Mutant adults exhibited prolonged survival and a reduced mortality rate compared with those in the wild type. All experiments were repeated for at least three times with similar results. (E) Expression levels of the GCRV S4 and S11 segments in liver tissue from wild-type and mutant strains on day 4 after infection. (F) Expression levels of the GCRV S4 and S11 segments in liver tissue from wild-type and mutant strains on day 7 after infection. 2026-09-29T17:29:22Z Image Figure 10.1371/journal.ppat.1014544.g002 https://figshare.com/articles/figure/_p_A_i_nhre3_i_homozygous_mutant_of_rare_minnow_exhibits_resistance_to_GCRV_p_/34026289 CC BY 4.0