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oai:figshare.com:article/34026219 2026-09-29T17:29:07Z category_1 category_4 category_8 category_21 category_272 category_873 category_39 category_46 category_61 category_69 category_132 portal_5 item_type_1 month_year_09_2026

<p>Oxidation of tannic acid by ten isolates.</p> Ali Seid (25138293) Fisseha Getachew (25138296) Marye Alemu (25138299) Birhanu Andualem (25138302) Biophysics Biochemistry Microbiology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Immunology Developmental Biology Inorganic Chemistry Infectious Diseases simultaneously becoming one purified whenever possible presumptive bacillus species physiological saline solution environment every day 42 &# 176 wet textile operations diluted test tube certain industrial level produces significant dosages ten bacterial isolates characterize extracellular laccase including dye centers crude laccase enzyme bacterial isolates could maximum laccase production contaminated textile wastewater decolorizing textile dyes bacterial isolates extracellular laccase laccase enzyme textile wastewater strain level serially diluted contaminated wastewater laccase production textile dye enzyme produced study could viable alternative red ). producing bacteria potential strain ph 7 nutrient agar molecular identification main sources living creatures information gathered highly harmful highly colorful high potential greater amount global economy environmental pollution employed since <div><p>Textile industries are important in both the global economy and our daily lives. They are, however, simultaneously becoming one of the main sources of environmental pollution in the world. It needs a greater amount of water for wet textile operations such as dyeing, and as a result, produces significant dosages of wastewater. Textile wastewater is highly colorful and contains dyes that are highly harmful to living creatures, and is discharged to the environment every day. The objective of the present study was to isolate and characterize extracellular laccase-producing bacteria from textile dye-contaminated wastewater to decolorize the dye. Samples were collected from the dye-contaminated wastewater sample site, including dye centers (DC), dye printing (DP), dye mixers (DM), and dye drains (DD). To isolate extracellular laccase producing bacteria from dye contaminated wastewater sample, 1 ml of sample was taken and serially diluted in physiological saline solution. Then, 0.1 ml from each diluted test tube was spread over nutrient agar and incubated. Then, the colonies were sub-cultured. Ten bacterial isolates were identify and designated as DMC1, DMC2, DMC3, DMG3, DMW1, DMY, DDY, DPG, DPC, and DCC. These bacterial isolates were tested for laccase production, and only four of them (DMC2, DMC3, DMG3, and DMW1) gave a positive result. Using morphological and biochemical characters, the four isolate were identified as a presumptive Bacillus species. Laccase production by isolates was optimized, and Maximum laccase production and dye decolonization were achieved at pH 7, a temperature of 42°C, and a dye concentration of 0.05 g per 50 mL of culture mediumTherefore, the enzyme produced, using the potential strain is capable of decolorizing textile dyes (black, yellow, and red). As a result, crude laccase enzyme from these bacterial isolates could be employed since a viable alternative to the physical and chemical processes of textile dye, as they have a high potential for decolorizing the dyes in this study. Scale up the production of laccase enzyme at certain industrial level is significant. Laccase enzyme should be purified whenever possible. Furthermore, molecular identification can be used to correctly identify up to strain level. The information gathered in this study could be used as a baseline for treating dye-polluted and contaminated environments.</p></div> 2026-09-29T17:29:01Z Image Figure 10.1371/journal.pone.0354516.g001 https://figshare.com/articles/figure/_p_Oxidation_of_tannic_acid_by_ten_isolates_p_/34026219 CC BY 4.0